Methods: Gradient PCR was employed in analysis of aldosterone synthase (CYP11B2) gene polymorphisms, and gel Imaging Technology was used to determine the optimal annealing temperature.
方法：采用梯度PCR对醛固酮合酶基因(CYP11B2)进行扩增，通过凝胶呈像技术判断CYP11 B2基因最佳退火温度。
The repressor gene and its allele-a temperature sensitive mutant have been cloned from the defective prophage PBSX of Bacillus subtilis by means of PCR technique.
本研究利用聚合酶链式反应技术，成功地克隆了枯草芽孢杆菌缺陷型原噬菌体PBSX阻遏基因及其温度敏感型等位基因。
At the same time temperature control is one of the keys of PCR technology, which is of PCR chip.
同时温度控制是PCR的关键技术之一，也即是PCR芯片的关键技术。
An array of miniature heaters corresponding to the array of PCR chips is made on a flexible printed circuit board that provides distributed temperature compensation to each PCR chip.
在柔性印刷电路板上形成与芯片阵列对应的微型加热器阵列，针对各芯片进行分散的温度补偿。
Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。
The cardinal temperature that PCR chips need is controlled by macroscopic control device, in which water as heat medium is pumped through a special heat exchanger.
宏观集中控制装置以水为传热媒质，通过特制换热器给芯片提供聚合酶反应所需的基本温度；
Objective:To explore a method for quicker identification of the optimal annealing temperature in PCR.
前言： 目的：探讨和尝试一种较为迅速确定最佳退火温度的方法来扩增目的基因。
PCR-chip is a function biochip for DNA polymerase chain reaction. The temperature control section is so important that it decides the performance, dimension, integration of the whole chip.
P CR芯片是进行DNA聚合酶链式反应的芯片，温控部分的搭构是决定整个PCR芯片性能、尺寸、集成化的重要组成部分。
A temperature control system for quantitive polymerase chain reaction (PCR) is presented in the paper with both software and hardware configuration.
从硬件和软件两方面定量pcr反应温度控制系统的设计。
Thermal analysis on the thermopneumatic pump to be used in the PCR chip is performed to understand the relation between chamber pressure and temperature field.
对热气动式微泵进行了热分析，得到了泵室压力与温度场的关系。
The third part is to use the sputtering, photolithography to produce heaters and temperature sensors which heat the reaction micro chamber and provide the temperature condition for the PCR reaction.
第三，用溅射、光刻等工艺在微型腔体底部制作微型加热器和温度传感器，实现对反应腔体的加热及其温度的精确测量，提供PCR扩增反应所需的温度条件。
Result: Compared with normal PCR, Gradient PCR could identify the the optimal annealing temperature of aldosterone synthase gene.
结果：与普通pcr相比，梯度PCR能够迅速地确定CYP11B2基因的最佳退火温度。
An integrated microstructure PCR-DNA amplification biochip based on MEMS technology was fabricated, which includes heater, temperature sensor, reaction chamber and inlet channels.
基于MEMS技术研制了微结构的集成PCR-DNA分子扩增生物芯片，芯片集成了加热子、温度传感器、反应室、进样通道等；
The analysis results prove that the designed chip has uniform temperature distribution and higher temperature rising and cooling rates and could meet the requirement of PCR.
分析结果表明设计的聚合物芯片温度分布均匀，有较高的升、降温速率，可以满足PCR反应的要求。
Because that the temperature detecting sensor and sample tubes are rotates on the same disk, the invention can control temperature accurately during PCR detecting.
本发明由于温度探测传感器与样品管在同一圆盘上作旋转运动，因此可达到PCR检测过程中样品控温准确的目的。
Because that the temperature detecting sensor and sample tubes are rotates on the same disk, the invention can control temperature accurately during PCR detecting.
本发明由于温度探测传感器与样品管在同一圆盘上作旋转运动，因此可达到PCR检测过程中样品控温准确的目的。
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