Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
方法将特异性引物从S8546病毒感染细胞PCR扩增的产物克隆于T载体，正确的克隆纯化后测序，应用DNASTAR软件比较分析。
Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.
方法设计特异的PCR引物，用RT-PCR技术分段扩增Q32株全长L、M片段，PCR产物纯化后直接测序，或用T-A克隆方法进行PCR产物克隆，然后进行遗传进化分析。
High-pure gene fragments could be obtained from little amount of sample through PCR and T-vector.
利用PCR反应和T载体可以从少量的标本中，高效获得高纯度的目的基因。
High-pure gene fragments could be obtained from little amount of sample through PCR and T-vector.
利用PCR反应和T载体可以从少量的标本中，高效获得高纯度的目的基因。
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