Because now there's minus t divided by RT squared.
现在又负T除以rt平方。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.
方法从Z 10株病毒感染的细胞提取总rna，将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定，应用dnastar软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
PCR and RT PCR showed that CD gene was transferred into the t lymphocyte and expressed successfully.
经pcr和RT PCR方法检测证明CD基因已成功地导入T淋巴细胞中并有效表达。
To study dysfunction of activated T cell in peripheral blood and make clear its role on pathogenesis in patients with recurrent tonsillitis(RT).
目的探讨反复扁桃腺炎（RT）患儿T细胞活化功能障碍情况，为分析RT的病因和发病机制提供理论依据。
The P and T values of male's CE were remarkably higher than those of female's, while of RT was lower in VCPT.
VCPT男性患儿虚报错误的T值、P值显著高于女性，女性患儿的平均反应时间T值和P值显著高于男性；
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
方法设计出针对各片段的特异性引物，用P CR方法从Z 37病毒感染的细胞提取细胞总rna，逆转录扩增、产物克隆t载体，纯化后测序，测定的序列应用DNASTAR软件比较分析。
Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
方法将特异性引物从S8546病毒感染细胞PCR扩增的产物克隆于T载体，正确的克隆纯化后测序，应用DNASTAR软件比较分析。
Further the earth pressure distribution with RB and rt modes was obtained using the same software, and the results were compared with t mode.
进一步研究了RB、RT模式挡土墙土压力的分布情况，并与T模式情况进行对比。
Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.
方法设计特异的PCR引物，用RT-PCR技术分段扩增Q32株全长L、M片段，PCR产物纯化后直接测序，或用T-A克隆方法进行PCR产物克隆，然后进行遗传进化分析。
Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.
方法设计特异的PCR引物，用RT-PCR技术分段扩增Q32株全长L、M片段，PCR产物纯化后直接测序，或用T-A克隆方法进行PCR产物克隆，然后进行遗传进化分析。
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