High-pure gene fragments could be obtained from little amount of sample through PCR and T-vector.
利用PCR反应和T载体可以从少量的标本中，高效获得高纯度的目的基因。
It has a hat because it's a unit vector, and t because it's tangent.
这上面有一个帽子表示它是单位矢量，T表示它是切线。
Well, to answer that we have an easy observation, which is that the vector from Q0 to Q of t is proportional to the vector from Q0 to Q1.
为了回答这个问题，我们观察可以知道，由Q0指向Q的那个向量,和由Q0指向Q1的向量成比例。
In this example, I create a vector (t) with values ranging from 0 to 2pi (with a step size of 0.2).
在这个例子中，先是创建了一个向量(t)，其值的范围从0到2pi(步进大小为0.2)。
r So, let's remember that the amount by which we moved, t delta r, is approximately equal to v times delta t, OK, and just using the definition of a velocity vector.
所以记住移动的量△,约等于v*△,这是用速度矢量的定义。
But creating detailed vector images takes patience and skill. You might be creating something as simple as an icon, or as cool as a T-shirt design.
但是创建一个繁琐的矢量图需要耐心和技术，你可能在创建一个简单的图标，或者是个很酷的T恤设计，如果有一批现成的矢量图会简化你的工作。
From free T-Shirt designs to stock vector images, the site has 7, 800 + free vector image files and packs.
从免费的T恤设计到常用的矢量图片，它包含了7800 +的免费矢量图片文件和打包文件。
For example, a monochrome vector of Bob Marley is just the thing for that T-shirt imprint. Or check out Clint Eastwood's sketch.
例如，鲍勃·马利(bob Marley)的黑白矢量图就是T恤图案，还有克林特·伊斯特伍德的素描矢量图。
Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
方法将特异性引物从S8546病毒感染细胞PCR扩增的产物克隆于T载体，正确的克隆纯化后测序，应用DNASTAR软件比较分析。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.
方法从Z 10株病毒感染的细胞提取总rna，将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定，应用dnastar软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Objective to assess the changes of t vector loop in children with Kawasaki disease (KD).
目的：探讨川崎病(KD)患儿空间t向量环变化。
T vector and their nucleotide sequences were analyzed.
载体，进行测序和序列分析。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
方法设计出针对各片段的特异性引物，用P CR方法从Z 37病毒感染的细胞提取细胞总rna，逆转录扩增、产物克隆t载体，纯化后测序，测定的序列应用DNASTAR软件比较分析。
A unit vector normal to the plane of t and n, which is the plane of curvature.
所构成的平面的单位向量，该平面为曲率所在的面。
Circle matrix is the algebraic description of all the resource circles in a directed graph, and equivalence reciprocally to that one of complementary set and T-characteristic vector.
回路矩阵是一个资源有向图全部资源回路的代数描述，并且它与补集及t -特征向量矩阵相互等价。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
When the price vector doesn t change along the idea changeable turnpike, we gives out the sufficent and neceseary condition about the price vector relative stability.
由分析可知，当价格向量沿理想变化路变化时，价格向量最稳定； 当价格向量沿非理想变化路变化时，给出了价格向量相对稳定的充要条件。
In vector control, there are the cross-coupling voltages between the m and t components of the stator current for Induction Motor (IM), it affects the performance of speed control system.
矢量变换后异步电动机数学模型中还存在M轴和T轴间的交叉电势的耦合作用，影响调速系统的控制性能。
In the paper online identification based on time window and least square support vector machine (LSSVM) for T-S model is proposed, including structure and parameter identification.
本文提出了基于时间窗的最小二乘支持向量机t - S模型在线辨识算法，包括结构辨识和参数辨识。
Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.
方法设计特异的PCR引物，用RT-PCR技术分段扩增Q32株全长L、M片段，PCR产物纯化后直接测序，或用T-A克隆方法进行PCR产物克隆，然后进行遗传进化分析。
Methods The L and M segment cDNA of Hantavirus Q32 strain was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pGEM-T Vector and then sequenced.
方法设计特异的PCR引物，用RT-PCR技术分段扩增Q32株全长L、M片段，PCR产物纯化后直接测序，或用T-A克隆方法进行PCR产物克隆，然后进行遗传进化分析。
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