Conclusion: Regions of apoptotic cells in "API method" and sub-G1 method were not the same. There is no coincidence in results of apoptotic percentage detected by "API method" and sub-G1 method.
结论:“API技术”和亚g 1峰检测法的细胞凋亡域不一致,“API技术”对细胞周期特异性凋亡的检测结果与亚g 1峰检测结果也不一致。
Results: (1) Sub-confluent cells having starved in DMEM containing 0.4% FBS for 48h, 88.5% of the cells were at G0/G1 phase of the cell cycle.
研究结果表明:(1)培养至次融汇状态的乳鼠心脏成纤维细胞经低血清(0.4%FBS)饥饿培养48小时,G0/G1期细胞占88.5%;
HL 60 and K562 cells showed typical apoptosis features of "ladder" and the sub G1 peak in DNA histogram.
作用HL60和K562细胞后电泳显示有凋亡细胞特有的“梯状”条带,DNA直方图出现亚g1峰。
HL 60 and K562 cells showed typical apoptosis features of "ladder" and the sub G1 peak in DNA histogram.
作用HL60和K562细胞后电泳显示有凋亡细胞特有的“梯状”条带,DNA直方图出现亚g1峰。
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