Sample stacking in capillary electrophoresis can concentrate sample species through the electrical field strength gradient caused by the inhomogeneous buffer concentration field in capillary.
毛细管电泳样品电堆积富集是一种通过缓冲溶液浓度的差异在毛细管中形成电场强度梯度,从而对样品进行浓缩的富集技术。
Methods: Based on several reported SDS-PAGE systems for low molecular weight protein, the buffer systems, concentrations of separating gel and stacking gel, electrophoresis procedures were optimized.
方法:比较以往报道的各种低分子量蛋白的电泳分离方法,对电泳缓冲液系统、分离胶和浓缩胶的浓度、电泳程序等进行了优化。
Methods: Based on several reported SDS-PAGE systems for low molecular weight protein, the buffer systems, concentrations of separating gel and stacking gel, electrophoresis procedures were optimized.
方法:比较以往报道的各种低分子量蛋白的电泳分离方法,对电泳缓冲液系统、分离胶和浓缩胶的浓度、电泳程序等进行了优化。
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