Objective: to establish a stable cell line that can be induced to express a target gene.
目的：建立一个可诱导表达目的基因的细胞系以研究新基因的功能。
Thus, the human embryo corneal epithelial cells was fit for establishing a relative stable cell line.
因此，人胚胎角膜上皮细胞适合于建立相对稳定的细胞系。
METHODS Natural products were collected to screen in the stable cell line of GLP-1 receptor, and the active component was identified in the PC12 cells.
方法建立稳定的GLP-1受体激动剂高通量筛选模型，从天然药物中筛选活性成分；在PC12细胞中验证GLP-1受体激动剂的功能及作用机制。
Objective: to establish a stable cell line and employ the cell line as a model to study target integration of exogenous cDNA and modulation of gene expression.
摘要目的：建立稳定整合的细胞株，为研究外源基因定位整合机制及其对靶细胞生物特征影响，建立一个生物模型。
Conclusion: a stable cell line expressing p37 regularly was established, which provides a good cell model for studying p37 function and its molecular mechanism.
结论已建立受四环素调控的p37高效表达细胞系，为进一步研究p 37的功能和作用机制提供了实验细胞模型。
Objective: to construct eukaryotic expression vector of human death receptor (DR5) and transfect NS-1 cells to establish stable NS-1 cell line.
目的：构建人死亡受体5 (DR5)真核表达载体，转染NS - 1细胞，建立稳定转染的NS - 1细胞系。
Objective:To establish a stable CHO cell line expressing GLUT4-EGFP, and to lay a foundation for investigating the translocation mechanisms of GLUT4 in CHO cells.
目的：建立稳定表达EGFP标记的葡萄糖转运蛋白4的CHO细胞系，为研究GLUT4在CHO细胞中的转运调节机制奠定基础。
Objective To construct eukaryotic expressing vector of human melanin-concentrating hormone receptor 1(MCHR1), then to transfect CHO cells with the vector for establishment of stable CHO cell line.
目的构建人黑色素浓集激素1型受体（MCHR1）真核表达载体，转染CHO细胞，建立稳定转染的CHO细胞系。
Objective To clone human epidermal growth factor receptor (HER2) gene and establish a MCF-7 cell line with stable co-expression of HER2 and estrogen receptor (ER).
目的克隆人表皮生长因子受体（HER2）基因，建立共表达HER2基因和雌激素受体（ER）基因的MCF-7细胞模型。
Objective: To establish highly effective and stable multidrug resistance (MDR) bladder cancer cell line.
目的：建立高效、稳定的人膀胱癌多药耐药性细胞模型。
Retrovirus vector; Stable transfected cell line; Stem cells.
逆转录病毒；稳定转染；干细胞。
Stable transfection of mouse B16 cell line can highly express G250, thus laying a foundation for the application of G250 in immunotherapy for tumors.
该稳定转染细胞系的建立为G250在肿瘤免疫治疗中的应用奠定了研究基础。
Objective To construct the human G250 eukaryotic expression vector and establish the stable B16 cell line expressing human G250 in mice.
目的构建人G250真核表达载体，建立稳定表达人G250的小鼠黑色素瘤细胞系。
Methods BTT-T739 cells, a mouse bladder transitional cell carcinoma cell line, were tranfected with GFP plasmid to screen stable GFP expressing clones.
方法 将绿色荧光蛋白 （GFP）表达质粒导入 膀胱癌细胞株BTT T739，筛选出高表达GFP的细胞克隆并配置成细胞悬液。
Methods BTT-T739 cells, a mouse bladder transitional cell carcinoma cell line, were tranfected with GFP plasmid to screen stable GFP expressing clones.
方法 将绿色荧光蛋白 （GFP）表达质粒导入 膀胱癌细胞株BTT T739，筛选出高表达GFP的细胞克隆并配置成细胞悬液。
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