The results of genotyping were compared with those obtained with the polymerase chain reaction method using sequence specific primers ( PCR-SSP).
并把分型结果与采用聚合酶链反应-序列特异性引物(PCR-SSP)获得的结果进行比较。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
方法运用聚合酶链反应-序列特异性引物(PCR-SSP)法,对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls.
方法利用聚合酶链反应-序列特异引物(PCR - ssp)法,对189例银屑病患者和273例健康人的HLA - DQA1和DQB1等位基因进行检测。
Conclusion PCR-SSP technology can exactly detect RHCE genotype in individuals of Han population in north China and Li population in south China.
结论P CR - SSP技术能准确判断中国北方汉族和南方黎族个体的RHCE基因型。
The KIR genotype of 50 patients with leukemia and 60 healthy controls in northern. Hans were analyzed by PCR-SSP.
应用PCR-SSP技术对北方地区50例白血病患者和60名健康对照者进行KIR基因的检测和分析。
Objective To establish DNA typing for HLA-DR antigens by polymerase chain reaction with sequence-specific primers (PCR-SSP).
目的采用顺序特异引物聚合酶链反应(PCR -SSP)建立人类白细胞抗原DR位点的DNA分型方法。
In this study, a nested PCR SSP and a direct amplification PCR SSP protocols for HLA DR genotyping were developed and were used in the selection of matched donor for sibling BMT.
该研究通过设计合成hla基因序列特异性引物,建立了HLA -DR基因分型的套式扩增和直接扩增pcr - SSP技术,并在骨髓移植配型中进行了应用。
HLA-DR2, DR7, DR9 genotyping by polymerase chain reaction with sequence-specific primers (PCR-SSP)was carried out for 35 individuals and 4 cell lines DNA.
采用顺序特异性引物聚合酶链式反应(PCR-SSP)DNA分型技术,首次对35例肾移植供受者和4份标准DNA进行HLA-DR2、DR7、DR9基因配型。
Objective To establish a method of high resolution DNA typing for HLA B40 cross reactive groups (CREG) in Chinese with polymerase chain reaction with sequence specific primers (PCR SSP).
目的采用顺序特异引物聚合酶链反应技术(PCRSSP),建立汉族人群HLAB40交叉反应组高分辨度dna分型方法。
DNA samples of related hematopoietic stem cell transplant donor-recipients were extracted from peripheral blood cells. HLA-A loci were typed both by RSCA and PCR-SSP.
然后分别提取84例拟进行造血干细胞移植病人及家系成员的外周血d NA,采用RSCA和序列特异性引物体外基因扩增(PCR ssp)法平行对照对HLA A基因位点进行分型。
DNA samples of related hematopoietic stem cell transplant donor-recipients were extracted from peripheral blood cells. HLA-A loci were typed both by RSCA and PCR-SSP.
然后分别提取84例拟进行造血干细胞移植病人及家系成员的外周血d NA,采用RSCA和序列特异性引物体外基因扩增(PCR ssp)法平行对照对HLA A基因位点进行分型。
应用推荐