PCR assay for identification of Babesia equi was developed by specific primers.
并利用特异性引物初步建立了马巴贝斯虫病PCR检测技术。
Based on the reported genomic RNA sequence of PLRV, two specific primers were synthesized.
根据已报道的马铃薯卷叶病毒基因组序列，设计合成一对特异性引物。
The specific primers were used to amplify some fungal pathogens of maize and healthy tissues.
表明此特异性引物可以用于玉米组织内南方锈菌的分子检测及病害的早期诊断。
Methods Sequence specific primers were designed and synthesized according to the HCV H strain of virus sequence.
方法根据HCVH病毒株序列设计、合成序列特异性的引物。
A pair of specific primers of gene encoding phenol hydroxylase was designed by oligonucleotide high conservative sequence.
根据苯酚羟化酶基因高度保守序列设计一对该基因的特异引物。
Objective To establish DNA typing for HLA-DR antigens by polymerase chain reaction with sequence-specific primers (PCR-SSP).
目的采用顺序特异引物聚合酶链反应（PCR -SSP）建立人类白细胞抗原DR位点的DNA分型方法。
Methods We used a hot initiated PCR based method with asset of universal acanthamoeba specific primers to detect acanthamoeba.
方法以一段特异性通用引物并配合热启动聚合酶链反应技术来检测临床标本中的棘阿米巴原虫。
Methods Specific primers for amplifying DC-SIGNR repeat regions were designed, and the PCR products were analyzed by electrophoresis.
方法设计特定引物，用PCR方法对DC-SIGNR基因绞链区重复序列进行扩增，用凝胶电泳法对其产物进行分析。
Methods: Serum samples from 160 cases with chronic HBV infection were collected and tested for HBV genotypes by type-specific primers.
方法采用多对型特异性引物-聚合酶链反应检测160例慢性乙型肝炎患者血清HBV基因型；
Mixed infections were tested with specific primers among 22 Malvastrum coromandelianum samples collected from several areas of Yunnan province.
对来自云南不同地区表现黄脉症状的22个赛葵样品进行了复合侵染分析。
The results of genotyping were compared with those obtained with the polymerase chain reaction method using sequence specific primers ( PCR-SSP).
并把分型结果与采用聚合酶链反应-序列特异性引物（PCR-SSP）获得的结果进行比较。
HLA-DR2, DR7, DR9 genotyping by polymerase chain reaction with sequence-specific primers (PCR-SSP)was carried out for 35 individuals and 4 cell lines DNA.
采用顺序特异性引物聚合酶链式反应（PCR-SSP）DNA分型技术，首次对35例肾移植供受者和4份标准DNA进行HLA-DR2、DR7、DR9基因配型。
Methods: By using polymerase chain reaction-sequence specific primers, frequency of HLADRB1 alleles in 72 patients with UC and 314 healthy controls were detected.
方法：采用序列特异性引物聚合酶链反应的方法对汉族72例uc患者和314例正常对照者HLA - DRB1基因分型。
Results: DNA samples from 70 healthy people and 43 patients with psoriasis were detected by PCR with 45 specific primers and two primers as internal quality control.
结果：本研究合成的45条特异性引物和两条内质控引物，检测70例健康人和43例银屑病患者DNA标本。
Conclusion: The study has successfully established a sensitive, accurate and fast, suitable for domestic application HBV genotype-specific primers PCR typing method.
结论：本研究成功建立了一种改良hbv型特异性引物pcr基因分型方法，其具有灵敏、准确、快速、经济、国内适用等特点。
After mixing the extracted nucleic acids with specific primers, the reaction was carried out under the certain temperature and then the electrophoresis test was made.
首先将萃取之核酸与特异性引子混合后，于特定温度下进行反应，随后再经电泳检测结果。
Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。
Methods:78 strains of clinically isolated staphylococci were detected by specific primers in conserved regions of mecA, ermA, ermC genes and compared with MIC determination.
方法 ：根据上述抗性基因保守区域选用引物经聚合酶链反应检测成都地区临床分离78株耐 药葡萄球菌与临床 药敏试验比较并追踪其预后。
Objective To establish a method of high resolution DNA typing for HLA B40 cross reactive groups (CREG) in Chinese with polymerase chain reaction with sequence specific primers (PCR SSP).
目的采用顺序特异引物聚合酶链反应技术(PCRSSP)，建立汉族人群HLAB40交叉反应组高分辨度dna分型方法。
Using the specific primers REA-FEA designed in this work by ourselves, we proved the specificity and sensitivity of the immuno-capture-PCR method again. The same result has been achieved.
利用本实验室自行设计的梨火疫病菌特异性引物REA-FEA，再次验证了免疫吸附-PCR技术的灵敏性和专化性，得到了同样的结果。
Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls.
方法利用聚合酶链反应-序列特异引物(PCR -ssp)法，对189例银屑病患者和273例健康人的HLA - DQA1和DQB1等位基因进行检测。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
White papers, Primers, etc. : contributed or solicited papers whose purpose is a call for action, a position paper, or an educational treatise on a specific issue.
白皮书、入门等：投稿或征稿，目的在于对特定问题呼吁行动、表明立场或教育性作品。
Electrophoresis analysis showed that 13 pairs of primers produced stable and specific bands(50%), and, of which 6 were polymorphic(23.1%).
结果表明，有13对引物能获得稳定的特异性条带（占总数的50%），其中有6个微卫星位点具有多态性（占总数的23.1%）。
Methods According to the specific sequence of APP genes, the primers and the fluorogenic probe were designed and synthesized.
方法根据APP的基因序列，设计并合成引物和荧光标记探针。
Then capture FMDV from clinical material with sterilized Eppendrof tubes of coating type-specific IgG, Amplify the conserved viral sequences by common primers (FM1/FM4).
在FMDV的核苷酸序列相对保守区设计上下游引物FM1/FM4，扩增病毒的基因片段。
The BOV97M and bovine 1.715 satellite DNA sequences were selected as the primers of male and bovine-specific DNA, respectively.
分别选择BOV97M做公牛特异扩增引物及1.715卫星DNA做牛特异扩增内标引物。
The new primers designed are highly specific and sensitive in detection of the two mites.
新设计的引物用于两种尘螨的检测有较好的特异性和敏感性。
After DNA elongation, such molecules can serve as a template for amplification and will be picked up in the final product, as will product generated by non-specific annealing of PCR primers.
颠末dna地断裂伸长率，这些分子能够作为模板扩增，将加速最终产物，如将产物所发生地非特异性退火地PCR引物。
After DNA elongation, such molecules can serve as a template for amplification and will be picked up in the final product, as will product generated by non-specific annealing of PCR primers.
颠末dna地断裂伸长率，这些分子能够作为模板扩增，将加速最终产物，如将产物所发生地非特异性退火地PCR引物。
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