The recombination fusion protein was purified by lysis, washing, denaturation and renaturation. The specific band of expression was excised from the gel and used to immunize mice .
通过裂解、洗涤、变性、复性等方法对包涵体蛋白进行处理，以电泳切胶回收所获得的纯化产物作为抗原，免疫小鼠制备抗重组蛋白血清。
The recombination fusion protein was purified by lysis, washing, denaturation and renaturation. The specific band of expression was excised from the gel and used to immunize mice .
通过裂解、洗涤、变性、复性等方法对包涵体蛋白进行处理，以电泳切胶回收所获得的纯化产物作为抗原，免疫小鼠制备抗重组蛋白血清。
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