Objective: to investigate the effect of the exercise on glucose transporter4 (GLUT4) gene expression and insulin binding in streptozotocin induced diabetic rat skeletal muscle.
目的:研究运动对链脲佐菌素所致的糖尿病大鼠骨骼肌细胞葡萄糖运载体4 (GLUT4)基因表达及胰岛素受体结合的影响。
Methods: 1. Primary rat skeletal muscle satellite cell was cultivated in vitro. The cultured cells were identified with cellular immunochemical stain.
方法:1体外分离培养大鼠骨骼肌卫星细胞,免疫组化鉴定骨骼肌卫星细胞并检测其纯度。
Energy can induce the expression of gene of UCP1 in rat BAT and UCP2 in WAT, but decrease the expression of gene of UCP3 in skeletal muscle.
说明能量能诱导大鼠褐色脂肪组织ucp1和白色脂肪组织ucp2基因表达,但降低骨骼肌ucp3基因的表达。
Results: Thedielectric response of the skeletal muscle cells from normal rat showed dependence of frequency, discrepancy in orientation, and two characteristic frequencies.
结果:正常大鼠骨骼肌细胞介电响应具有频率依存性,存在方向性差异,其介电弛豫现象表现为两个特征频率。
Objective To investigate the effect of resistin and resistin binding peptide(RBP) on the glucose uptake in L6 rat skeletal muscle cells.
目的探讨抵抗素及其结合多肽(RBP)对大鼠骨骼肌细胞糖摄取功能的影响。
Rat triceps brachii muscle was acquired to separate skeletal muscle satellite cells with the two-step method of collegenase-1 and trypsin and were cultured and subcultured in vitro.
取大鼠肱三头肌,采用二步消化法分离骨骼肌卫星细胞,体外进行原代培养和传代培养。
Rat triceps brachii muscle was acquired to separate skeletal muscle satellite cells with the two-step method of collegenase-1 and trypsin and were cultured and subcultured in vitro.
取大鼠肱三头肌,采用二步消化法分离骨骼肌卫星细胞,体外进行原代培养和传代培养。
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