Objective: To quickly detect two pathogenic bacteria strains of food poisoning by single strand conformation polymorphism (SSCP) assay.
目的:利用单链构象多态性(SSCP)对两起食物中毒的致病菌进行快速分析。
Single strand conformation polymorphism (SSCP) essay and sequence analysis of the PCR product were used to ascertain the gene mutation.
方法应用PCR及单链构象多态性(SSCP)分析技术结合基因序列测定方法确定突变类型。
Methods:Polymerase chain reaction (PCR) and single strand conformation polymorphism(SSCP) was used.
方法:聚合酶链反应(PCR)、单链构象多态性(SSCP)分析。
Objective: To observe the effect of different gel preparation on detecting mutation of AKT2 gene by single-strand conformation polymorphism (SSCP).
目的:观察单链构象多态性(SSCP)筛查akt2基因突变时凝胶配制的不同对筛查结果的影响。
PCR and single strand conformation polymorphism analysis (SSCP) were combined with DNA sequencing confirmation to screen all 28 exons of SCN5A gene.
采用PCR单链构象多态性技术(SSCP)结合DNA序列测定证实,对病人SCN5A的全部2 8个外显子进行突变检测。
PCR and single strand conformation polymorphism analysis (SSCP) were combined with DNA sequencing confirmation to screen all 28 exons of SCN5A gene.
采用PCR单链构象多态性技术(SSCP)结合DNA序列测定证实,对病人SCN5A的全部2 8个外显子进行突变检测。
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