Methods Serum free cells suspension culture and single cell cloning technique were used to isolate neuroepithelial stem cells.
方法利用无血清悬浮培养、单细胞克隆技术分离神经上皮干细胞；
Cloning, molecular --- Transfer of a DNA sequence into a single cell of a microorganism, followed by culture of the microorganism to produce large quantities of the DNA sample for analysis.
将DNA序列转移到微生物单细胞中，然后培养微生物，以产生大量DNA样本用以分析。
Cloning takes the DNA from a single cell to create a whole new individual.
克隆能单个细胞的DNA创造出一个完整的新个体。
Cloning of these single cells then allowed the creation of a whole animal in which the gene was knocked out in every cell.
克隆这些单细胞然后再将其培育成一只完整的动物，这样动物身上的每个细胞中的这种基因就都被去除了。
Cloning of these single cells then allowed the creation of a whole animal in which the gene was knocked out in every cell.
克隆这些单细胞然后再将其培育成一只完整的动物，这样动物身上的每个细胞中的这种基因就都被去除了。
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