Methods:The techniques including G banding, karyological analysis, PCR amplification and silver staining were used.
方法：应用染色体G分带、核型分析和PCR及银染技术。
After systematically study on the main factors involved, an AFLP silver-staining analysis system suitable for walnut genomic DNA was established.
通过对内切酶用量、酶切时间、连接时间、稀释倍数等的研究，建立了适于核桃分析的AFLP银染技术体系。
Methods immunohistochemistry in 52 cases of nasopharyngeal carcinoma PTEN gene expression, the use of PCR SSCP silver staining, DNA sequencing analysis to detect PTEN gene mutation in exon 5, 8.
方法采用免疫组化检测52例鼻咽癌中pten基因的表达，利用PCRSSCP银染、DNA测序分析等方法检测pten基因第5、8外显子突变。
Methods immunohistochemistry in 52 cases of nasopharyngeal carcinoma PTEN gene expression, the use of PCR SSCP silver staining, DNA sequencing analysis to detect PTEN gene mutation in exon 5, 8.
方法采用免疫组化检测52例鼻咽癌中pten基因的表达，利用PCRSSCP银染、DNA测序分析等方法检测pten基因第5、8外显子突变。
应用推荐