It is prepared by activating bacteria, shake-flask culturing, expanding propagating and mixing.
经菌种活化、摇瓶培养、扩繁，混合制得。
We prepared 6.58mg soluble MGL from 1L shake-flask culture with higher activity, with a 41.1% overall yield in the purification process.
活性测定显示可溶性MGL具有较高活性，每升发酵菌液可以制备6.58毫克可溶性MGL，纯化回收率达到41.1%。
Objective To investigate the influences of oxygen vector n-hexadecane on Xanthophyllomyces dendrorhous growth and astaxanthin production in the shake-flask culture.
目的考察氧载体正十六烷对红法夫酵母发酵产虾青素的影响。
Shake-flask fermentation under optimal conditions showed that glucoamylase secreted by the transformant GB0506 was 17.5% higher than parental strain F0410 at the end of fermentation.
对转化子进行摇瓶发酵研究，发酵终止时转化子GB0506的糖化酶活力比出发菌株F0410提高了17.5%。
Addition of propanol during erythromycin fermentation with in shake flask improved the titer by 49.
在摇瓶培养过程中，发酵初期加入正丙醇可使红霉素的效价提高49。
The optimum composition of culture medium for erythromycin fermentation with maize meal hydrolysate instead of glucose in the shake flask was studied.
研究了摇瓶试验中玉米粉水解物替代葡萄糖发酵红霉素的最佳培养基组成。
Firstly, transgenic kelp gametophyte cells were cultured in the shake flask to find the growth characteristic of gametophytes.
首先，摇瓶悬浮培养配子体细胞，考察配子体细胞的生长特性。
Shake flask culture was used and the yield of polysaccharide was taken as main index to ensure the optimum culture conditions of submerged fermentation for Ganoderma lucidum by corn mash medium.
以灵芝多糖为指标，采用摇瓶液体发酵的方法，研究了以玉米水解糖为主要原料，液体培养灵芝的发酵条件。
The production of erythromycin in the shake flask was significantly influenced by adding different kinds of organic nitrogen source (e. g. amino acids).
红色糖多孢菌的摇瓶培养基中使用不同有机氮源时红霉素的效价明显不同。
The composition of culture media, and the cultivation condition of shake flask process had great effects on the nutritional ingredient of mycelium.
摇瓶液体培养所用培养基成分及营养含量以及培养条件的改变影响菌丝体的营养成分。
Remove the needles and shake the flask to dislodge the solid from the bottom and to help to dissolve it.
拔去针头，摇动烧瓶，把固体从瓶底取出，而且把它溶解。
The flask shake experiment showed that the strain NBT was capable of not only releasing potassium from soil minerals but also absorbing most of the potassium released.
摇瓶试验表明，NBT菌株不仅能分解土壤矿物并释放出其中的钾，而且具有很强的吸钾功能。
Through shake flask fermentation, the effects of microwave radiation, the exogenous gentamicin concentration, concentration of NaCl and some other factors on the secretion of gentamicin were studied.
研究了外加抗生素、胞外庆大霉素浓度、微波处理、培养基中氯化钠浓度等因素对于细菌庆大霉素分泌的影响。
Through shake flask fermentation, the effects of microwave radiation, the exogenous gentamicin concentration, concentration of NaCl and some other factors on the secretion of gentamicin were studied.
研究了外加抗生素、胞外庆大霉素浓度、微波处理、培养基中氯化钠浓度等因素对于细菌庆大霉素分泌的影响。
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