Thus, the serum medium can't be used as ESCs medium without feeder.
因此，在没有饲养层的条件下，表皮干细胞的培养必须添加条件培养基。
The wear volume of bone cement samples in the calf serum medium is larger than that in the air and increases with the contact load increase.
骨水泥试样的磨损量在小牛血清介质中比在空气中大，并且随接触载荷增大而增大。
In calf serum medium, the wear mechanism of fretting is mainly adhesive wear, fatigue wear and the weakening effect of solution molecules on the bone cement matrix under stress.
骨水泥在小牛血清介质中微动磨损的损伤机制主要为黏着磨损和疲劳磨损，溶液分子在应力作用下对骨水泥基体有削弱作用。
Presumptive zygotes were cultured in mSOFaa medium supplemented with OCS or FBS had higher blastocyte yield than that in culture medium with free serum.
受精卵在含血清FBS或OCS培养液中的囊胚发育率明显高于在无血清培养基中的囊胚发育率。
Conclusion: it is identified that the splenocytes culture supernatant has growth factor like action and can substitute for serum in the medium.
结论：推测梅花鹿脾细胞培养上清具有生长因子样活性，并在细胞培养基中代替血清的作用。
Cell line GLC surviving and proliferation was observed in medium containing 5% calf bovine serum.
细胞株glc可在含5%小牛血清的培养基中存活和增殖。
Albumin, serum in the culture medium decreased the transfection efficiency.
培养液中的白蛋白、血清可降低转染效率。
Objective to explore the optimal condition of direct differentiation into dopaminergic neurons of embryonic stem cells in serum-free and feeder layer cell free medium.
目的寻求无血清、无饲养层细胞存在的情况下，胚胎干细胞向多巴胺能神经元定向诱导分化的最佳条件。
METHODS: the influence of he ne laser irrigation on the growth and development of neurons in hippocampus and cortex of frontal lobe neurons was observed int he cell culture of serum free medium.
方法：采用无血清培养的新生大鼠海马神经元和额前叶皮质神经元，观察氦氖激光照射对神经元生长发育的影响。
Bovine serum is a common component in medium of cell and tissue culture.
小牛血清是细胞组织培养中常用的培养基成份。
The first experiment approach adopted the addition of the rabbit's serum to the culture medium while in the second experiment the CXZP was added to the medium directly.
第一种采用给家兔灌服血府逐瘀浓缩丸后取其血清加至细胞培养基中进行实验即血清药理学方法；第二种直接加血府逐瘀煎剂浓缩液至培养基中。
Results The results showed that the yield, titer and purity of those McAbs were 2-4 times higher than those produced in serum containing medium.
结果无蛋白培养基中单抗的产量与效价比有血清培养的单抗高2～4倍，而且单抗的纯度显著提高。
Objective To investigate the influence of physiological saline and human blood serum as the dilution medium on direct bilirubin detection with vanadate oxidation method.
目的探讨生理盐水与人血清做稀释介质对钒酸盐法测定直接胆红素的结果影响。
The results showed that GMTB containing 2% serum and 0.2% glucose was the most optimum medium.
结果表明，用含有2%犊牛血清、0.2%葡萄糖的改良马丁肉汤培养基较为合适；
BM cells were cultured in medium containing autologous serum for 3 weeks to expand adherent MSC.
骨髓细胞在含有自体血清的培养基中培养3周为了扩增贴壁的间充质干细胞。
The lymphocytes were isolated with separating medium and then erythrocytes and serum were collected.
血液分离淋巴细胞，并收集红细胞和血清。
After 3 passages were obtained, and then digested by DMEM medium containing fetal calf serum, cell amount was counted and the proliferation curve of MSCs was depicted.
取第3代骨髓基质细胞，以含胎牛血清的DMEM培养液培养消化后，计数细胞量，描绘细胞增殖曲线。
Conclusions CD medium is able to replace serum containing medium and very suitable for production of high quality McAb.
结论无蛋白培养基可完全取代常规的有血清培养基，用于生产高滴度、高纯度单克隆抗体。
This paper suggested that KSOM can be used as serum free medium for ovine embryo development in vitro.
说明KSOM可以用于绵羊胚胎早期发育的无血清培养液。
During the later period of the cultivating process, the applying of serum free medium had no significant effects on the cell growth and protein expressing.
在培养后期换用无血清培养基培养，细胞生长及蛋白表达水平正常。
The higher serum UA level had relation with serious disease situation, the serious patients group was higher than light-medium group with very significant situation(P<0.01).
血清UA水平的高低与病情严重程度相关，危重度组高于轻中度组，差异有显著意义（P<0·01）。
It was demonstrated that pituitary cells could survival and synthesize and release hormones in serum-free medium.
结果表明分散垂体细胞在无血清培养液中能够存活，并具有合成和分泌激素的功能。
Methods Human keratinocytes were primarily cultured in vitro with serum free medium.
方法以无血清培养基体外原代培养角质形成细胞。
Objective To develop a simple serum-agar slant medium for cultivation and isolation of tubercle bacilli.
目的提出一个可供分离培养结核杆菌的简易血清琼脂斜面培养方法。
We used embedding medium to cover lipid of pumpkin seed, emulsifier and stabilizer to settle delamination of pumpkin seed serum, the effect of powder was fairly good.
采用包埋剂包裹瓜子仁中的油脂，乳化剂、稳定剂解决瓜子浆中蛋白质和脂肪的分层问题，获得了理想的成粉效果。
Results: The spiral ganglion neurons could survive and had a normal phenotypic differentiation in serum-free medium.
结果：显示耳蜗螺旋神经节神经元在体外无血清培养条件下，可以存活并进行正常分化。
Conclusion At present, the serum-containing medium for culture of CHO cells can not be completely replaced by serum-free serum.
结论目前无血清培养基尚不能完全取代含血清培养基用于培养CHO细胞。
AIM to observe the effects of epidermal growth factor (EGF) and bovine pituitary extract (BPE) on human pituitary adenoma cells cultured in serum free medium.
目的观察表皮生长因子(EGF)和牛垂体提取物(BPE)在人垂体腺瘤细胞培养中的作用。
Methods: We selected the best medium fitted for es cells culture, and analyzed the neural differentiation of es cells by comparing increased number of viable cells among different serum-free medium.
方法：比较ES细胞在不同培养基中的生长情况，分析ES细胞在不同时间分化形成神经细胞的比例。
The model of dissociated cell cultures of human fetal pituitary in serum-free medium was established.
采用人胎脑垂体前叶分散细胞建立了无血清体外培养模型。
应用推荐