We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.
经设计通用引物、PCR扩增、克隆和测序，首次从分子水平鉴定了杂草赛葵上的双生病毒。
Methods Molecular cloning, sequencing and bioinformatics techniques were used to identify the difference between the new HLA allele and other known alleles.
方法采用基因克隆、测序及生物信息学技术，分析HLA新等位基因与HLA已知基因序列的差异。
Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。
Methods Molecular biology technologies of RT-PCR, directed cloning and DNA sequencing were carried out.
方法采用RT -PCR、定向克隆和DNA测序等一系列分子生物学技术进行本实验。
The zebrafish genome sequencing project has played important roles in mutant mapping and cloning, and comparative genomic research projects.
斑马鱼基因组测序计划在突变图谱与克隆，及比较基因组研究计划中扮演着重要的角色。
Objective Molecular cloning and sequencing of the human nerve growth factor gene.
目的克隆人神经生长因子基因编码区。
ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
通过引物设计、P CR、基因克隆、测序，获得了10种鸢尾属植物和1个外类群种的ITS序列。
The purified DNA can be used directly in subsequent enzymatic reactions such as labeling, sequencing, cloning, and ligation, as well as for PCR analysis.
纯化的DNA可直接用于后续的应用，如标记、测序、克隆、连接和PCR分析。
Aim: Cloning and partially sequencing of human dentin matrix protein 1 (DMP1) encoding mature protein.
目的：克隆人牙本质基质蛋白1 (DMP1)成熟肽编码区基因片段。
Aim: Cloning and sequencing of hOP-1 cDNA encoding mature protein.
目的：克隆成骨蛋白-1（OP-1）成熟蛋白编码区基因。
To generate the recombinant antibodies, the key point is the cloning and the sequencing of the VH and VL genes from antibody secreting hybridoma cell line.
而要研制基因工程抗体，鼠源性抗体可变区基因的克隆与测序是关键。
Aim: Cloning and partially sequencing of mouse dentin sialoprotein (DSP) encoding mature protein.
目的：克隆小鼠牙本质涎蛋白(DSP)成熟肽编码区基因。
On the basis of cloning and sequencing of OPF02 757 , two PCR primers were designed and a genome specific PCR marker for H.
在对OPF0 2 757进行克隆、测序的基础上，设计一对PCR引物，建立了簇毛麦基因组特异性PCR标记。
To study the genomic characteristics of IBV variant isolate, we obtained the full genome sequences of nephropathogenic JH06111 by amplifying, cloning and sequencing of its cDNA.
为研究IBV变异株的基因组及其变异特征，本实验通过基因扩增拼接的方法获得了肾病变型毒株JH06111的全基因组序列。
An efficient degrader Nocardia sp. C-14-1 was isolated from acrylic fiber wastewater. The identification, cloning and sequencing of a gene encoding nitrilase from strain C-14-1 was introduced.
从腈纶废水中分离到高效降解多种污染物的诺卡氏菌株C-14-1，并对该菌中腈水解酶的基因进行鉴定和测序。
An efficient degrader Nocardia sp. C-14-1 was isolated from acrylic fiber wastewater. The identification, cloning and sequencing of a gene encoding nitrilase from strain C-14-1 was introduced.
从腈纶废水中分离到高效降解多种污染物的诺卡氏菌株C-14-1，并对该菌中腈水解酶的基因进行鉴定和测序。
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