METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
方法运用序列特异性引物聚合酶链反应技术，检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
Control DNA samples that genotypes known were used to confirm the sensitivity and specificity of each sequence-specific primer.
引物的特异性和灵敏度采用基因型已知的质控DNA进行验证。
Control DNA samples that genotypes known were used to confirm the sensitivity and specificity of each sequence-specific primer.
引物的特异性和灵敏度采用基因型已知的质控DNA进行验证。
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