So this semi-nested RT-PCR could be used for rapid detection of the muscovy duck reovirus disease.
因此，该半套式RT-PCR可以用于番鸭呼肠孤病毒病的临床快速检测。
Methods: TTV-DNA of 113 cases of parturients' blood serum and neonatal umbilical blood were detected by semi-nested polymerase chain reaction.
方法：应用半巢式聚合酶链反应技术检测113例孕产妇血清及其新生儿脐血的TTV—DNA。
Therefore, the rapid, sensitive and stable semi-nested PCR are the effective methods for detecting trace bovine and sheep derived materials in feedstuff.
该技术具有快速、灵敏和结果稳定的特点，是检测饲料中痕量牛羊源成分的一种有效方法。
Compared with the conventional PCR, semi-nested PCR appeared to be more sensitive and more practicable, thus providing a reliable method to detect M. suis.
结论本试验建立的半巢式PCR诊断方法具有特异、敏感、实用等特点，为猪附红细胞体的检测提供了一种可靠的诊断方法。
Although using nested or semi-nested PCR can prominently increase PCR specificity, it really cannot efficiently amplify quick evolving region of DNA in our research of gene Fak56D.
虽然巢式和半巢式PCR等能显著提高扩增的特异性，而应用于高变异区的扩增结果仍是杂带多或涂抹严重，不能满足后续实验需要。
A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee (Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.
基于中蜂囊状幼虫病病毒基因组序列AF469603中的RNA依赖的RNA聚合酶基因，建立该病毒的半套式RT-PCR检测方法。
Relations are flat, highly structured, strongly typed, and unordered while XML data is ordered, nested, hierarchical, optionally typed and often irregular and semi-structured.
关系型数据是平面的、高度结构化、强类型化、无序的，而XML数据是有序、嵌套的、层次化的、可选类型化的，并且往往不规则且是不完全结构化的。
Amberfish is general purpose text retrieval software. It supports nested queries of semi-structured text in XML format and traditional unstructured searching.
格式的半结构文本的嵌入式查询以及传统的机构型的搜索。
Methods:HGV RNA was detected by a semi nested RT PCR.
方法：通过半巢式逆转录多聚酶链反应检测HGV。
Methods:HGV RNA was detected by a semi nested RT PCR.
方法：通过半巢式逆转录多聚酶链反应检测HGV。
应用推荐