Results: the linear amplification range of PCR and the best cycle number and template of RT-PCR were obtained.
结果：获取了PCR线性扩增范围，确定了RT - P CR的最佳循环次数和模板量。
Conclusion RNA can be extracted from the OCT-embedded long-stored tissues treated in 25% sucrose in PBS. And all the extracted RNA can be used to RT-PCR amplification.
结论从25%蔗糖磷酸缓冲液固定oct包埋的冰冻时间较长的组织标本中可提取组织rna，并用于进行RT - PCR扩增。
Conclusion RNA can be extracted from the OCT-embedded long-stored tissues treated in 25% sucrose in PBS. And all the extracted RNA can be used to RT-PCR amplification.
结论从25%蔗糖磷酸缓冲液固定oct包埋的冰冻时间较长的组织标本中可提取组织rna，并用于进行RT - PCR扩增。
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