These RT-PCR products were sequence verified.
这些RT - PCR扩增产物的序列验证。
The level of promiscuous gene expression was detected by RT-PCR.
利用RT - PCR方法检测小鼠胸腺异位基因表达水平。
Mutation analysis was detected by direct sequencing RT-PCR products.
直接测序法检测RT PCR产物的基因变异；
We endeavored to detect animal viruses in water primarily by RT-PCR.
同时也对水体中动物病毒的检测作了初步探索。
MethodsReverse Transcription-polymerase chain reaction(RT-PCR) was used.
方法采用逆转录-聚合酶链反应（RT-PCR）方法。
Methods RT-PCR and in situ hybridization histochemistry (ISHH) were used.
方法rt - PCR和原位杂交组织化学技术。
Methods Total RNAs of tumor tissues were extracted and were detected by RT-PCR.
方法提取肿瘤组织总rna，用反转录聚合酶链式反应(RT - pcr)法进行检测。
One step nested reverse transcriptase-polymerase chain reaction (RT-PCR) was used.
使用一步法巢式逆转录聚合酶链扩增法(RT - PCR)。
Methods Neural stem cell culture, Immunocytochemistry and RT-PCR technique were used.
方法神经干细胞培养，免疫细胞化学及RT - P CR技术。
Viral detection by reverse transcription polymerase chain reaction (RT-PCR) assay, and.
采用逆转录聚合酶链式反应(RT - PCR)检测病毒。
Methods RT-PCR was used to examine the expression of M2 and M3 in clasp fibers and sling fibers.
方法应用rt - PCR方法，研究钩状纤维和套索纤维中M 2、M3的分布与表达。
So this semi-nested RT-PCR could be used for rapid detection of the muscovy duck reovirus disease.
因此，该半套式RT-PCR可以用于番鸭呼肠孤病毒病的临床快速检测。
The intracellular pattern recognition receptor NOD1 is detected in human lung epithelial cells by RT-PCR.
并用RT-PCR的方法检测肺上皮细胞胞内模式识别受体NOD1的表达。
The results of semi-quantitative RT-PCR experiments supported the reliability of our microarray analysis.
半定量反转录-聚合酶链反应验证了芯片分析结果的可靠性。
The present paper analyzed the expression of MSTN gene in different organs of Big Bone Chicken by RT-PCR.
以大骨鸡为试材，采用RT-PCR法检测了MSTN基因在大骨鸡不同器官中的表达情况。
Conclusions Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR.
结论应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
The RT-PCR was adopted to detect the expressions of resistin gene in epiploon and subcutaneous fatty tissues.
采用反转录-聚合酶链反应检测两组大鼠大网膜和皮下脂肪组织中抵抗素基因的表达。
Results: the linear amplification range of PCR and the best cycle number and template of RT-PCR were obtained.
结果：获取了PCR线性扩增范围，确定了RT - P CR的最佳循环次数和模板量。
The results of RT-PCR analysis for 7 differently expressed genes were coincident with those of microarray assay.
PCR技术对其中7个基因表达差异的验证结果与基因芯片结果一致。
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
The result indicated that fluorogenetic quantitation RT-PCR may be used as a good reference in detection of H5N1 TIV.
这说明荧光定量RT-PCR方法可以对临床上H5N1虎源流感病毒检测提供参考。
Myrosinase activity was detected in all the tested organs except for pulp, which is consistent with the result of RT-PCR.
酶活性测定表明，除了果肉外的其它器官中都有芥子酶活性，这与RT-PCR的结果是一致的。
During the procedure, different molecular biology techniques were involved such as PCR RT-PCR and Yeast-two-hybrid system.
在试验过程中用到了很多高级的生物学技术，比如说PCR,RT - PCR和酵母双杂交技术。
Expression of osteoblast specific genes, chondrocyte specific genes, and adipocyte specific genes were confirmed by RT-PCR.
成骨细胞、软骨细胞以及脂肪细胞特异相关或标志基因的表达用RT - P CR检测。
Analysis of RT-PCR and sequencing indicated that the ncRNA gene had two different transcripts through alternatively splicing pattern.
PCR和测序结果分析，发现这个非编码RNA基因通过选择性剪接产生两种不同的转录产物。
Analysis of RT-PCR and sequencing indicated that the ncRNA gene had two different transcripts through alternatively splicing pattern.
PCR和测序结果分析，发现这个非编码RNA基因通过选择性剪接产生两种不同的转录产物。
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