Methods (1) The coding sequence of MG7 mimotope (GC23) was included in the reverse primer, and by PCR incorporated with HSP70 gene at the 3 terminus.
方法1。根据HSP70全长基因序列设计针对其编码区的引物，在下游引物的宁端加人MG7模拟表位的核昔酸序列。
A TC-RT-PCR method basing on reverse transcription with random primer facilitated the detection for those samples mixed infected by ASGV and ACLSV.
用随机引物反转录，通过TC-RT-PCR检测ACLSV和ASGV复合感染的梨样品也均获得了目标片段。
Its RNA polymerase activity was dependent upon the primer in vitro with limited reverse transcription activity.
其RNA依赖的RNA聚合酶活性有一定的引物依赖性。
Its RNA polymerase activity was dependent upon the primer in vitro with limited reverse transcription activity.
其RNA依赖的RNA聚合酶活性有一定的引物依赖性。
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