Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.
用离子交换柱层析法分离,用反相高效液相色谱法进一步纯化,经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
AIM to establish a reverse phase high-performance liquid chromatography (RP-HPLC) method to determine the level of tolbutamide in human plasma.
目的:建立反相高效液相色谱法测定人血浆中甲磺丁脲含量的方法。
Recombinant human BDNF was expressed in E. coli and is supplied in a lyophilized form. A greater than 96% purity was determined by reverse phase-HPLC and SDS-PAGE.
重组人脑源性神经营养因子(BDNF)在大肠杆菌中表达,以冻干形式提供。反相高效液相色谱法和SDS-PAGE测定其纯度大于96%。
Methods The fluorospectrophotometry and reverse phase high performance liquid chromagraphy(RP HPLC).
方法荧光分光光度法和反相高效液相色谱法。
The title constants were determined by reverse-phase HPLC on an ODS column with methanol as mobile phase.
提出用反相高效液相色谱法测定文题常数。
Then the pretreated samples were extracted with acetonitrile, further cleaned up by reverse solid phase dispersion, and analyzed by reversed phase HPLC with diode array detector (DAD).
韭菜样本用微波炉加热处理,使酶钝化消除含硫基质干扰,然后用乙腈提取、逆固相分散净化,用反相高效液相色谱-二极管阵列检测器检测。
LDTI was extracted by acetone and purified by reverse-phase high performance liquid chromatography(HPLC).
采用丙酮抽提LDTI并用反相高效液相纯化。
The contents of hyoscyamine and scopolamine in the seeds of Hyoscyamus Niger were determined by applying reverse phase ion-pair HPLC.
本文用离子对HPLC法测定了天仙子中莨菪碱和东莨菪碱的含量。
The contents of hyoscyamine and scopolamine in the seeds of Hyoscyamus Niger were determined by applying reverse phase ion-pair HPLC.
本文用离子对HPLC法测定了天仙子中莨菪碱和东莨菪碱的含量。
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