Retrovirus vector; Stable transfected cell line; Stem cells.
逆转录病毒；稳定转染；干细胞。
Objective To investigate the effectivness of recombined retrovirus vector in gene therapy.
目的探索逆转录病毒载体在基因治疗乙型肝炎中的应用。
Objective to construct antisense and sense vimentin retrovirus vector for investigating the effect of vimentin on reactive gliosis.
目的构建反义及正义波形蛋白重组逆转录病毒载体，研究波形蛋白在反应性胶质化中的功能与作用。
The results demonstrate that self-inactivating retrovirus vector can be used for gene transcription regulation research during the aging process.
结果表明，自我失活型逆转录病毒载体能很好地用于衰老基因转录调控的研究。
Methods a retrovirus vector (RV) which contains JAK2 gene and two binding sites for a chemical inducers of dimerization (AP20187) was constructed.
方法构建克隆1个含有JAK2基因和二聚化化学诱导物(AP2 0 187)结合位点所组成的逆转录病毒载体。
Conclusion the retrovirus vector containing the PIG-A gene was successfully constructed and the expression of CD59 on the mutant K562 cells could be increased.
结论成功构建了PIG - A基因逆转录病毒载体，感染突变型k562细胞后可以提高其CD59的表达。
AIM: to study the effect of calcitonin gene related peptide (CGRP) on contraction and relaxation of iliac artery transduced with CGRP gene by retrovirus vector.
目的：探讨降钙素基因相关肽(CGRP)基因转导兔动脉内膜对其舒缩功能的影响。
Conclusion HumanPD-1 gene has been cloned, recombinant retrovirus vector containing PD-1 gene constructed and L929 transfection cell line expressing PD-1 molecules selected.
结论构建了含人pd - 1基因重组逆转录病毒的载体，筛选出稳定表达人PD - 1分子的L929细胞株。
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
Retrovirus (RV) is the most widely used vector for gene transfer, but its efficiency is very low for human cells.
逆转录病毒是最常用的基因治疗载体，但对人类细胞的基因导入率较低。
Retrovirus (RV) is the most widely used vector for gene transfer, but its efficiency is very low for human cells.
逆转录病毒是最常用的基因治疗载体，但对人类细胞的基因导入率较低。
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