Objective To construct saccharomyces cerevisiae expression vector with GFP as report gene.
目的构建以绿色荧光蛋白(GFP)为报告基因的酿酒酵母表达载体。
Results Saccharomyces cerevisiae expression vector with GFP as report gene was constructed and expressed successfully.
结果成功构建了以GFP为报告基因的酿酒酵母载体,并在酵母中得到表达。
Objective To investigate the use of albumin-coated microbubbles targeted delivery report gene by therapeutic ultrasound-mediated microbubble destruction.
目的探讨白蛋白包裹微泡在治疗超声照射下对基因传输的作用。
Using Agrobacterium vacuum infiltration method the plant bivalent expression vector pBI121 containing GUS as a report gene was transformed into lettuce Lactuca sativa l.
以GUS基因作为报告基因,利用农杆菌真空渗透法,采取正交试验设计对莴苣LactucasativaL。
ConclusionUsing GFP as a report gene in eukaryotic cell transfection, the lipofectamine method could get a higher transfection efficiency and higher survival rate for cells.
结论采用绿色荧光蛋白为报告基因的真核细胞转染技术中,脂质体法是效率高、安全性大的方法。
Methods:One of the three internal translation initiation region(TIR) was chemically synthesized and cloned into the report plasmid, and the expression of the report gene was studied.
方法:将基因内翻译起始序列合成后克隆到上游含有起始密码和无起始密码的报告质粒中,研究表达水平的差异。
Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.
目的构建携带报告基因增强型绿色荧光蛋白(EGFP)的反转录病毒载体,并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。
The main task for post-doctorate is molecular imaging research for hepatic diseases, especially in the fields of report gene, plasmid construction, and monocrystalline iron oxide nanocompound (MION).
肝脏疾病的分子影像学研究,注重于报告基因、质粒构建和纳米单晶磁性粒子的研究。
According to a report published online in the journal Nature, researchers have used gene therapy to allow color-blind squirrel monkeys to look at their fruit in a whole new light.
自然周刊网上刊登的一篇报道称,科学家们已经应用基因疗法使色盲的松鼠猴们能够以全新的视角看它们的水果。
By comparing the DNA of Japanese with each type, the researchers were able to identify the gene that controls which type a person has, they report in the Monday issue of Nature Genetics.
通过比较两种类型耳垢的日本人的DNA,研究人员鉴定出了控制耳垢类型的基因,研究结果刊登在周一发布的《自然遗传学》期刊上。
Timothy Walsh with Cardiff University in Britain, who first uncovered the gene and wrote the Lancet report, worried that experts did not know how wide the bug had spread in India.
英国加迪夫大学的TimothyWalsh是《柳叶刀》上报告的作者,也是首个发表文章揭露这种基因的学者。他担忧专家们并不知道这种病毒在印度的泛滥。
Myles Axton, the editor of the journal that is publishing the report, said he was not persuaded by the argument that the dry earwax gene had been favored by natural selection.
刊登这份报告的期刊编辑MylesAxton说他并不认为干态耳垢基因是自然选择的结果。
In the May 29th issue of Cell, scientists report discovering a gene that controls fruit flies' sensitivity to ethanol.
在预期于5月29日出版的《细胞》杂志上,科学家将报告一种新基因的发现,这种基因能够让果蝇远离酒精。
In a June 2005 report, IBM Blue Gene/L clocks at 183.5 tflops (trillion flops).
在 2005 年6 月的报告中,IBMBlueGene/L 的时钟峰值为 183.5tflop(万亿flop)。
However, the current Nature Genetics report associates SLE with a polymorphism at gene TNFSF4.
然而,当期《自然-遗传学》杂志报告sle与TNFSF4基因多态性有关。
It might be possible to superpower the gene, known as PTEN, by tinkering with an enzyme that regulates its activity, scientists report in the Jan. 12 issue of Cell.
据1月12日的《细胞》杂志上的一篇研究报告,有可能通过修补一种可调节pten活性的酶来加强其基因的表达。
The study is actually the first to report an example of a childhood cancer caused by mutations in a cancer-causing gene.
这项研究实际上是第一个报道一例因基因突变引起的儿童癌症。
The results suggest that gene silencing may be "an efficient and more specific approach for therapy of liver fibrosis, " the report states.
报告指出,得到的结果表明基因沉默可能是“一个有效的和更具体的治疗肝纤维化的方法”。
The patients who had two working copies of the BDNF gene were no more likely to develop childhood onset obesity than the general population, and did not report unusually high levels of overeating.
有两条正常BDNF基因的患者与普通人群相比,不太可能在儿童期开始发胖,也没有报告不正常的超常的暴食。
The angiotensin (AGT) gene polymorphisms M235T and T174M are associated with mortality in heart failure (HF) patients, New Zealand researchers report.
新西兰科学家报道:他们发现血管紧张素(AGT)多基因型M235T和T174M与心力衰竭患者的死亡率有密切关系。
We report the identification of a splice donor mutation in a novel gene, which is the mouse ortholog of a newly discovered telomeric regulator.
我们报告了一个新基因左拼接剪接变异的证据,这个新基因是一个新发现的鼠同源基因端粒调节体。
Here, we report the identification of the SUPERNUMERARY BRACT (SNB) gene controlling the transition from spikelet meristem to floral meristem and the floral organ development.
本文我们分离到了一个控制小穗分生组织向花分生组织转变以及花器官发育的基因SUPERNUMERARYBRACT (SNB)。
It is the first report of obtaining partial sequences of plant disease resistance gene by using antibody probe in the world, which provides a basis of cloning the whole gene encoding API protein.
在国内外首次利用抗体探针获得了植物抗病蛋白编码基因的部分序列,为进一步克隆全长的API蛋白基因奠定了基础。
This report summarized the researching status of wheat exogenous gene transformation in recent years. The prospects of its applications in wheat breeding field was also analysed.
笔者综述了近年来外源基因导入小麦的研究概况,阐述了外源基因导入的技术以及检测方法,分析了外源基因转化在小麦育种中的发展前景。
The report, given in mid-July at the International Conference on Alzheimer's Disease, concentrated on DNA surrounding the ApoE gene.
这份陈诉在7月中旬召开的国际阿尔茨海默氏症会议上提交处理,它集中研究了围绕着ApoE基因的DNA。
The report, given in mid-July at the International Conference on Alzheimer's Disease, concentrated on DNA surrounding the ApoE gene.
这份陈诉在7月中旬召开的国际阿尔茨海默氏症会议上提交处理,它集中研究了围绕着ApoE基因的DNA。
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