The recent research progress of recombinant protein refolding was reviewed.
本文对近年来重组蛋白复性技术的研究进行了评述。
Recently the refolding studies have been concentrated on alkaline phosphatase from E.
对小牛肠碱性磷酸酶再折叠的机制及其影响因素进行了探讨。
Protein refolding by dilution is the basis of research on other method of renaturation.
稀释复性法是研究其他方法的基础。
Then the purification protein was refolded according to the Merck protein refolding kit.
纯化后的蛋白按照墨克蛋白复性试剂盒说明书进行了复性。
The experimental results of urea gradient electrophoresis show that there are two different refolding processes.
尿素浓度梯度电泳实验结果显示,存在两条不同的折叠途径。
The possibility of peripherin binding protein (PBP) to improve the refolding of denatured luciferase in vitro was investigated.
探讨感光受体外周蛋白结合蛋白(PBP)在体外是否有促进变性蛋白质复性的作用。
The unfolding and refolding of bovine serum albumins and the existence of intermediates were studied by fluorescence phase diagram.
荧光相图法研究牛血清白蛋白变性和复性过程,捕捉部分折叠中间态。
Objective The possibility of peripherin binding protein (PBP) to improve the refolding of denatured luciferase in vitro was investigated.
目的探讨感光受体外周蛋白结合蛋白(PBP)在体外是否有促进变性蛋白质复性的作用。
The mechanism of oxidative refolding of proteins was elucidated in more detail from the intensive and extensive studies in the past decades.
经过近几十年来广泛而深入的研究,蛋白质氧化重折叠的机制已得到相当详细的阐明。
In this chapter solute retention mechanism in LC, protein refolding mechanism and new developments in protein refolding kinetics were reviewed. It contains 61 references.
本章对色谱中的保留机理及理论、蛋白质液相色谱复性机理及蛋白折叠动力学理论模型研究进展进行了综述,共包括文献61篇。
Methods Based on the reactive principle of molecular chaperone, we adopted an artificial molecular chaperone-assisted refolding method for recombinant endostatin refolding.
方法根据人工分子伴侣对重组蛋白质的复性机理,采用人工分子伴侣方法应用于重组内抑素的复性。
When the denatured-reduced egg white lysozymes were diluted by the dilution refolding buffer solution, an observable amount of aggregate precipitates would immediately be formed.
当用复性缓冲液稀释还原脲变性溶菌酶时,会迅速产生可观量的沉淀。
Then according to experiment design, the effects of rChIL-18 protein refolding yield at different densities were investigated by the systems of artificial chaperone at different densities.
然后按照试验设计,考察不同浓度组成的人工分子伴侣体系对不同浓度鸡IL-18重组蛋白复性率的影响。
Most of the proteins examined so far proceed oxidative refolding via multiple pathways rather than a single and specific pathway. This is consistent with the folding energy landscape theory.
在已研究过的蛋白质中,大多数蛋白质都是沿着多途径而非单一、特定的途径进行氧化重折叠,这与折叠能量景观学说是一致的。
Most of the proteins examined so far proceed oxidative refolding via multiple pathways rather than a single and specific pathway. This is consistent with the folding energy landscape theory.
在已研究过的蛋白质中,大多数蛋白质都是沿着多途径而非单一、特定的途径进行氧化重折叠,这与折叠能量景观学说是一致的。
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