The recombinant plasmid was transformed into E.
用化学法把重组质粒转化到E 。
The transformation of recombinant plasmid and screening;
重组质粒的转化及筛选；
Then the target recombinant plasmid was transferred into e.
然后阳性重组质粒转化入e。
Expression products of recombinant plasmid mainly located in nucleus.
重组质粒表达产物定位于细胞核；
Results The recombinant plasmid with mutant gene was constructed correctly.
结果突变基因重组表达质粒构建正确；
Objective: To find a rapid method of screening positive recombinant plasmid.
目的：建立一种快速筛选阳性重组质粒的方法。
Objective To construct recombinant plasmid for expression of chymopapain in E.
目的构建木瓜凝乳蛋白酶的表达载体，并在大肠杆菌中表达。
Objective:To study the curative effect of EAE with DT390-IL2 recombinant plasmid.
目的：研究DT390-IL2重组质粒对实验性变态反应性脑脊髓炎（EAE）的治疗效果。
The recombinant plasmid was transfected into the cell line HL-60 by electroporation.
采用电转染方法将重组体导入HL-60细胞中；
And then the recombinant plasmid and empty plasmid was delivered by intravenous injection.
经静脉分别注入重组质粒及空白质粒。
Then the recombinant plasmid was transfected into NK-92 cells with Hpofectamine encapsuled.
然后，通过脂质体转染法将其转入NK-92细胞；
The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body.
对含人EC-SOD重组质粒的工程菌进行诱导表达形成包涵体。
Objective:To construct the recombinant plasmid of HPV16L1-E7C and study its cellular immunity.
目的：构建HPV16L1-E7C重组质粒并研究其细胞免疫性。
Supercoiling is necessary for the expression of recombinant plasmid DNA at transcription level.
超螺旋化对于质粒dna在转录水平上的表达是必需的。
Tto identify whether the construction of recombinant plasmid. is successful by PCR and sequencing.
经pcr和测序的方法鉴定重组质粒是否构建成功。
The recombinant plasmid was confirmed by digestion and sequence, then transformed into E. coli BL21.
重组质粒经酶切和测序证实正确，然后转化大肠杆菌BL21。
Objective To construct a recombinant plasmid for expression of rabies virus glycoprotein in COS-7 cells.
目的构建狂犬病病毒糖蛋白基因的真核细胞表达载体，并在COS - 7细胞中表达。
The supernant of HCC cells transduced with the recombinant plasmid can support the growth of TF 1 cells.
表达产物能分泌到肝癌细胞外，分泌到细胞外的产物用TF-1细胞检测证明具有生物学活性。
Objective to optimize the selective method to obtain the pure recombinant plasmid transfected U937 cells.
目的：探索优化重组质粒转染细胞后的筛选方法，以得到高纯度U 937转染细胞。
Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
目的：消减文库构建过程中，用P CR技术快速筛选重组阳性克隆。
Objective To construct a recombinant plasmid containing rhoptry protein 2 (ROP2) gene of Toxoplasma gondii .
目的构建弓形虫棒状体蛋白2 （ROP2 ）基因重组质粒。
The recombinant plasmid pMM2 contained cholera enterotoxin B sub-unit gene CT-B were expressed in E. coli strain.
本实验室使霍乱肠毒素（CT）B亚单位基因在大肠杆菌中获得表达，并能分泌到胞外。
Recombinant plasmid PDSBCM, microorganisms transformed therewith, and method for producing alkaline protease VAPK.
重组质粒pdsbcm，转化微生物，产生一种碱性蛋白梅vapk的方法。
The specific masculine brown-yellow color reaction was seen in the muscle fibers of recombinant plasmid injected group.
免疫组化染色后在重组质粒注射部位肌肉组织的肌纤维内显示阳性的棕黄色分泌颗粒。
After the reading frame was confirmed correct by sequencing, the recombinant plasmid was transformed into expression host e.
测序表明读码框架正确后，将重组子分别转入表达宿主菌e。
After co-transfection of recombinant plasmid and wild type AcNPV DNA, the rAcNPV was confirmed by the end point dilution assay and dot blot.
经同源重组后，以终点稀释和斑点杂交法筛选重组杆状病毒；
The proliferation of recombinant plasmid in packaging cells (PT67) would be studied further as well as the transducing effect to the target cells.
我们将对此重组质粒在包装细胞（PT67）的扩增以及对靶细胞转导的效应作进一步研究。
Results Gel stained with coomassie blue G-250 showed that the induced E. coli carrying recombinant plasmid can produce 38 000 protein at high level.
诱导的工程菌抽提包涵体，以确定38 000蛋白质抗原在大肠杆菌中的表达形式。
Results: the constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45 - 4b基因。
Results: the constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45 - 4b基因。
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