An indirect ELISA was developed and optimized with the purified recombinant E2 protein as coating antigen. The optimal conditions of ELISA were determined as follows.
将表达的重组E2蛋白纯化后作为ELISA包被抗原，建立了一种基于重组E2蛋白的间接ELISA方法，并对此方法的反应条件进行了优化。
An indirect ELISA was developed and optimized with the purified recombinant E2 protein as coating antigen. The optimal conditions of ELISA were determined as follows.
将表达的重组E2蛋白纯化后作为ELISA包被抗原，建立了一种基于重组E2蛋白的间接ELISA方法，并对此方法的反应条件进行了优化。
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