Conclusion: Under adequate culture conditions, high density and high expression culture of the recombinant cell line could be realized by using bioreactor.
结论:在较为适当的培养条件下,利用生物反应器可使工程细胞的培养达到高密度、高表达。
They could also use recombinant DNA to study cell differentiation and development, including such cellular abnormalities as cancer.
他们还可能利用重组合DNA来研究细胞分化和发育,包括像癌症这样的组胞变态。
Conclusion: Recombinant expression vector PGL3-DF3-DTA could produce specific lethal effect on human breast cancer cell line of DF3 positive.
结论:重组表达载体PGL3 - DF 3 - DTA能对DF 3阳性的乳腺癌细胞产生特异性杀伤作用。
Objective: To study the effects of prokaryotically expressed recombinant human NDRG2 on tumor cell lines.
目的:考察原核表达的重组人NDRG2蛋白对肿瘤细胞的作用。
The recombinant protein could specially react with B7 2 McAb and could stimulate T cell proliferation combined with anti CD3 antibody.
在抗人CD3单抗的协同下,B72胞外区重组蛋白能有效地促进外周血单核细胞的增殖。
Cell density cultivation and high level expression of recombinant urease a fusion protein in Helicobacter pylori.
目的高密度培养重组细菌和高效表达重组幽门螺杆菌尿素酶a融合蛋白。
Conclusion HumanPD-1 gene has been cloned, recombinant retrovirus vector containing PD-1 gene constructed and L929 transfection cell line expressing PD-1 molecules selected.
结论构建了含人pd - 1基因重组逆转录病毒的载体,筛选出稳定表达人PD - 1分子的L929细胞株。
Objective to construct recombinant human leptin mammalian cell expression vector, and to express recombinant human leptin in COS-7 cells.
目的构建重组人瘦素哺乳细胞表达载体并在COS 7细胞表达重组人瘦素。
Objective To observe the effects of recombinant augmenter of liver regeneration (ALR) on the proliferation of hepatic stellate cell line IG12 and ECM synthesis.
目的观察重组大鼠肝再生增强因子(alr)对肝星状细胞株ig12增殖及细胞外基质合成的影响,探讨其抗肝纤维化作用的可能机制。
The relationship between virulence and the size of the plaques of recombinant vaccinia viruses was studied by plaque assay method on chicken embryo cell monolayer with AGAR overlay.
采用单层琼脂蚀斑测定法,初步研究了重组痘苗病毒的蚀斑大小与毒力之间的关系。
Conclusion the recombinant expression vector PGL3-DF3-DTA can produce a specific killing effect on the DF3-positive human breast cancer cell line.
结论重组表达载体PGL3 -DF 3 - DTA能对DF 3阳性的乳腺癌细胞产生特异性杀伤作用。
Cell factor is biological preparation capable of promoting wound heal and tissue regeneration and may be either natural or gene recombinant.
细胞因子是能够促进伤口愈合和组织再生的生物制剂,它可以是天然提取或基因重组。
The plasmid stability studies showed that recombinant plasmid has excellent segregational stability but the structural stability was not good in the host cell.
对重组菌中重组质粒的稳定性进行研究,结果表明该质粒在宿主菌中具有良好的分离稳定性,而结构稳定性较差。
Disclosed are useful constructs and methods for the expression of proteins using primary translation products that are processed within a recombinant host cell.
公开的是有用的构建体和方法,用于蛋白质的表达,使用重组宿主细胞内加工的初级翻译产物。
Optogenetics combines recombinant DNA technology and optic technology, and is very powerful for cell biology research.
光遗传学结合了重组DNA技术与光学技术,对细胞生物学的研究非常有用。
The invention relates to a recombinant DNA molecule coding a polypeptide having phytase activity after being expressed in a prokaryotic or eukaryotic host cell.
本发明涉及一重组DNA分子,其在原核宿主细胞或真核宿主细胞 中表达之后编码具有肌醇六磷酸酶活性的多肽。
Objective To investigate the localization of recombinant CLIC 1 protein in cultured cell line with its overexpression.
目的利用重组的胞内氯离子通道蛋白clIC1蛋白的过表达来了解该蛋白质在细胞中的定位。
The optional high cell-density fermentation conditions, purification methods and biological activity of the recombinant enterokinase in E.
对重组肠激酶在大肠杆菌中的高密度发酵表达条件、纯化方法及其生物活性测定进行了研究。
AIM: to investigate the inhibitory effects of recombinant human Mullerian inhibiting substance on cell proliferation in human ovarian carcinoma cells (OVCAR8 and SKOV3 cell lines).
目的:探讨重组人苗勒抑制物对人卵巢癌细胞株ovcar8及SKOV3细胞生长的影响。
Objective:To construct a recombinant defective retroviral vector carrying hNT3 gene and a stable virus-producing packaging cell lines , and then to identify them.
构建并鉴定携带人NT - 3基因的逆转录病毒载体以及稳定的包装细胞系。
Objective To analysis the therapeutic effects of pterygium treated by Recombinant Bovine Basic Fibroblast Growth Factor in Eye Drop Form with the bottom limbal stem cell autograft transplantation.
目的分析贝复舒滴眼液联合下方自体角膜缘干细胞移植术治疗翼状胬肉的临床疗效。
To generate the recombinant antibodies, the key point is the cloning and the sequencing of the VH and VL genes from antibody secreting hybridoma cell line.
而要研制基因工程抗体,鼠源性抗体可变区基因的克隆与测序是关键。
The recombinant plasmid was transfected into the cell line HL-60 by electroporation.
采用电转染方法将重组体导入HL-60细胞中;
The recombinant plasmid was transfected into the cell line HL-60 by electroporation.
采用电转染方法将重组体导入HL-60细胞中;
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