Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
结果建立的实时荧光定量PCR方法的灵敏度为10-4水平。
Real time quantitative PCR has been widely used in various areas of plant pathology research.
实时荧光定量pcr广泛应用于植物病理学研究的各个领域。
The real time quantitative PCR is applied to research the expression trend of these genes under five different hormones.
并通过荧光定量PCR研究其在外施五种激素的条件下的胶乳中的表达谱。
Objective: to develop an internal quality control substance of HBV-DNA by real time quantitative PCR and to evaluate its clinical value.
目的：制备h BV - DNA荧光定量pcr检测的室内质控物，并对其应用价值进行初步评价。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
Finally, the expression levels of the candidate genes were further confirmed by quantitative real-time PCR using a new set of samples (20 narcolepsy-cataplexy patients and 20 healthy controls).
最后，用定量实时PCR法在一组新样本(20名发作性睡病-猝倒患者和20名健康对照)中进一步确认候选基因的表达水平。
Methods The expression of TUSC3 gene in 20 cases of pancreatic cancer and 6 cases of normal pancreas were detected by oligonucleotide microarray and real-time quantitative PCR.
方法利用寡核苷酸基因芯片技术和实时定量pcr技术检测20例胰腺癌组织和6例正常胰腺组织中TUSC3基因的表达。
Cytology on BKV infection was carried out and was compared with PCR. Cytology has less sensitivity and specificity than real-time quantitative PCR.
开展了BK病毒感染细胞学研究，并与PCR方法作了比较，认为细胞学检查灵敏度和特异度均低于定量pcr法。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Objective To set up a duplex quantitative real-time PCR (QPCR) assay with high sensitivity, specificity and rapidity to detect Candida krusei and Candida glabrata.
目的建立一种快速、灵敏、特异的鉴定克柔念珠菌和光滑念珠菌的双重实时荧光定量PCR方法。
DNA methodology and gestational age had the largest effects on test performance, with real-time quantitative polymerase chain reaction (RTQ-PCR) outperforming conventional PCR.
所用的DNA方法学和所处的妊娠期对检测精度有着最大的影响，实时定量多聚酶链反应(RTQ - PCR)的检测精度超过常规的PCR。
Article on the principle of real-time fluorescent quantitative PCR, factors and plant diseases and genetic breeding of the application were reviewed.
文章对实时荧光定量pcr的原理、影响因素以及在植物病害和遗传育种方面的应用等进行了综述。
Methods Duplications or deletions of?PMP22gene were detected in 113 CMT cases, 4 HNPP cases and 50 normal controls by using real-time quantitative PCR. Results (Thirty-six) of 113 CMT cases had the?
方法采用实时荧光定量PCR检测113个腓骨肌萎缩症家系先证者、4个遗传性压力易感性神经病家系先证者和50名正常人PMP22基因重复或缺失突变。
To filter out some of the differential genes, 18 cases of AS group and 6cases of healthy control group are validated by real-time fluorescence quantitative PCR.
对筛选出的部分差异基因，选取病例组18例及正常对照组6例，以实时荧光定量PCR进行验证。
Uses real-time fluorescence quota PCR the method to carry on the quantitative analysis.
采用实时荧光定量pcr的方法进行定量分析。
Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.
目的：建立诺如病毒的荧光定量PCR检测方法，应用于急性胃肠炎的快速检测。
Differentially expressed genes were identified by real-time quantitative PCR.
采用实时荧光定量PCR鉴定高表达基因。
Multiplex PCR and real-time PCR are developed for simultaneous and quantitative detection of bacteria-producing biogenic amine.
利用多重PCR和实时定量PCR技术可以实现对葡萄酒中生物胺产生菌的快速定量检测。
Methods:Viral RNA Was extracted from feces. 92 samples were detected by real-time fluorescent quantitative PCR and Enzyme-linked Immunosorbent Assay(ELISA).
方法：提取粪便中的RNA，用实时荧光PCR方法对丽水市急性胃肠炎的92份标本进行检测，并与常规ELISA检测结果比较。
Objective To increase the sensitivity of quantitative detection of gene transcripts in peripheral blood using real time RT PCR, two specimen processing methods were compared.
目的选择合理的标本处理方法，提高外周血基因转录本检测的灵敏度。
In addition, the differences of these genes were studied by real-time quantitative PCR which in two soybean lines and different seed developmental stages.
此外，还利用荧光定量pcr技术进一步分析了这六个基因在两个大豆品种中的定量差异。
To establish a TaqMan-based real-time fluorescent quantitative PCR assay for detection and quantitation of Hepatitis E virus.
目的建立灵敏、稳定、特异的实时荧光PCR方法，用于戊型肝炎病毒的定量检测。
Results:Norovirus was successfully detected from epidemic sample of Lishui, the positive rates of real-time fluorescent quantitative PCR and ELISA were 78.26% and 55.43%, respectively.
结果：用实时荧光PCR方法成功的从丽水疫情样本中检测出诺如病毒，实时荧光PCR和ELISA的检出率分别为78.26%和55.43%。
Objective The serum HBV-DNA has been extensively detected by fluorescence-quantitative real time PCR (FQ-PCR) in our country.
目的目前国内较多的医院采用荧光定量的方法对血清中的HBV - DNA进行定量检测。
System real time quantitative RT-PCR technology and immunohistochemistry were used to detect the Chlamydia trachomatis urogenital tract, the expression of UU.
结果：①实验组女性泌尿生殖道沙眼衣原体、解脲支原体的表达高于对照组。
System real time quantitative RT-PCR technology and immunohistochemistry were used to detect the Chlamydia trachomatis urogenital tract, the expression of UU.
结果：①实验组女性泌尿生殖道沙眼衣原体、解脲支原体的表达高于对照组。
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