Differentially expressed genes were identified by real-time quantitative PCR.
采用实时荧光定量PCR鉴定高表达基因。
Cytology on BKV infection was carried out and was compared with PCR. Cytology has less sensitivity and specificity than real-time quantitative PCR.
开展了BK病毒感染细胞学研究，并与PCR方法作了比较，认为细胞学检查灵敏度和特异度均低于定量pcr法。
In addition, the differences of these genes were studied by real-time quantitative PCR which in two soybean lines and different seed developmental stages.
此外，还利用荧光定量pcr技术进一步分析了这六个基因在两个大豆品种中的定量差异。
Methods The expression of TUSC3 gene in 20 cases of pancreatic cancer and 6 cases of normal pancreas were detected by oligonucleotide microarray and real-time quantitative PCR.
方法利用寡核苷酸基因芯片技术和实时定量pcr技术检测20例胰腺癌组织和6例正常胰腺组织中TUSC3基因的表达。
DNA methodology and gestational age had the largest effects on test performance, with real-time quantitative polymerase chain reaction (RTQ-PCR) outperforming conventional PCR.
所用的DNA方法学和所处的妊娠期对检测精度有着最大的影响，实时定量多聚酶链反应(RTQ - PCR)的检测精度超过常规的PCR。
Methods Duplications or deletions of?PMP22gene were detected in 113 CMT cases, 4 HNPP cases and 50 normal controls by using real-time quantitative PCR. Results (Thirty-six) of 113 CMT cases had the?
方法采用实时荧光定量PCR检测113个腓骨肌萎缩症家系先证者、4个遗传性压力易感性神经病家系先证者和50名正常人PMP22基因重复或缺失突变。
Conclusion myocardial microcirculation in syndrome X could be quantitative analyzed by real-time myocardial contrast echocardiography.
结论实时心肌声学造影可定量分析x综合征患者心肌微循环灌注情况。
Finally, the expression levels of the candidate genes were further confirmed by quantitative real-time PCR using a new set of samples (20 narcolepsy-cataplexy patients and 20 healthy controls).
最后，用定量实时PCR法在一组新样本(20名发作性睡病-猝倒患者和20名健康对照)中进一步确认候选基因的表达水平。
A quantitative real-time polymerase chain reaction test provides rapid and accurate diagnosis of cytomegalovirus and improved management of immunocompromised patients.
一种定量实时多聚酶链反应能够快速准确的诊断巨细胞病毒，改善免疫抑制患者的治疗。
This system can not only automatically conduct quantitative loading, but also display, set and real - time monitor load process.
系统不但能自动完成定量装载，而且还可以对装载量进行显示、设定，对装载过程进行动态检测。
Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
结果建立的实时荧光定量PCR方法的灵敏度为10-4水平。
Objective: to develop an internal quality control substance of HBV-DNA by real time quantitative PCR and to evaluate its clinical value.
目的：制备h BV - DNA荧光定量pcr检测的室内质控物，并对其应用价值进行初步评价。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Objective To set up a duplex quantitative real-time PCR (QPCR) assay with high sensitivity, specificity and rapidity to detect Candida krusei and Candida glabrata.
目的建立一种快速、灵敏、特异的鉴定克柔念珠菌和光滑念珠菌的双重实时荧光定量PCR方法。
The real time, on-line, quantitative testing is offered for system of wire rope destruction, the method of determinant for wire rope destruction is better scientific, nicety, reasonable.
该系统可以实时、在线、定量的检测钢丝绳的损伤，对损伤的判定较目前的手段更为科学、准确、合理。
Article on the principle of real-time fluorescent quantitative PCR, factors and plant diseases and genetic breeding of the application were reviewed.
文章对实时荧光定量pcr的原理、影响因素以及在植物病害和遗传育种方面的应用等进行了综述。
Objective: To assess the value of Real time myocardial contrast echocardiography (MCE) in the quantitative evaluation of canine normal myocardial perfusion.
目的：探讨实时心肌声学造影定量评价犬正常心肌灌注的可行性。
To filter out some of the differential genes, 18 cases of AS group and 6cases of healthy control group are validated by real-time fluorescence quantitative PCR.
对筛选出的部分差异基因，选取病例组18例及正常对照组6例，以实时荧光定量PCR进行验证。
Uses real-time fluorescence quota PCR the method to carry on the quantitative analysis.
采用实时荧光定量pcr的方法进行定量分析。
Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.
目的：建立诺如病毒的荧光定量PCR检测方法，应用于急性胃肠炎的快速检测。
Based on improvement of fuzzy qualitative simulation algorithm, a new semi-quantitative and real-time fault prediction method of a class of dynamic system with incomplete information is proposed.
在对模糊定性仿真算法改进的基础上，提出了一类信息不完整动态系统的实时半定量故障预报方法。
The real time quantitative PCR is applied to research the expression trend of these genes under five different hormones.
并通过荧光定量PCR研究其在外施五种激素的条件下的胶乳中的表达谱。
Multiplex PCR and real-time PCR are developed for simultaneous and quantitative detection of bacteria-producing biogenic amine.
利用多重PCR和实时定量PCR技术可以实现对葡萄酒中生物胺产生菌的快速定量检测。
Methods:Viral RNA Was extracted from feces. 92 samples were detected by real-time fluorescent quantitative PCR and Enzyme-linked Immunosorbent Assay(ELISA).
方法：提取粪便中的RNA，用实时荧光PCR方法对丽水市急性胃肠炎的92份标本进行检测，并与常规ELISA检测结果比较。
Objective To increase the sensitivity of quantitative detection of gene transcripts in peripheral blood using real time RT PCR, two specimen processing methods were compared.
目的选择合理的标本处理方法，提高外周血基因转录本检测的灵敏度。
Real time quantitative PCR has been widely used in various areas of plant pathology research.
实时荧光定量pcr广泛应用于植物病理学研究的各个领域。
Results:Norovirus was successfully detected from epidemic sample of Lishui, the positive rates of real-time fluorescent quantitative PCR and ELISA were 78.26% and 55.43%, respectively.
结果：用实时荧光PCR方法成功的从丽水疫情样本中检测出诺如病毒，实时荧光PCR和ELISA的检出率分别为78.26%和55.43%。
Critical hemodynamic therapy is a goal-directed, quantitative treatment process based on hemodynamic theories in accordance with the patients real-time status and response to intervention.
重症血流动力学治疗是以血流动力学理论为基础，根据机体的实时状态和反应，目标导向的定量治疗过程。病因治疗是这个过程重要的、不可分割的组成部分。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
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