Objective To observe the effect of the poly dl lactide (PDLLA) used as cell culturing scaffold on the growth of rabbit chondrocytes.
目的观察国产外消旋聚乳酸(PDLLA)材料为细胞载体对软骨细胞吸附生长增殖的影响。
Methods Coated PLA, PGA, PLGA with poly-l-lysine, free chondrocytes isolated from rabbit articular cartilage were seeded onto three kinds of scaffolds after expansion by in vitro culture.
方法多聚赖氨酸包埋PLA，PGA，PLGA三维细胞支架。分离培养兔关节软骨细胞，体外扩增后种植到三种支架中。
OBJECTIVE: to build a method of differentiation of rabbit BMSCs into chondrocytes in vitro and to determine the factors involved in the differentiation process.
目的：体外培养兔骨髓间充质干细胞并定向诱导分化为软骨细胞，探讨体外诱导分化为软骨的方法和条件。
Methods Rabbit BMSCs were cultured in vitro induced to chondrocytes, and then the biologic characters of the chondrocyte were observed and tested.
方法体外培养获取生长状态良好的兔骨髓间充质干细胞，进行成软骨诱导，观察并检测其生物学特性。
Methods Rabbit BMSCs were cultured in vitro induced to chondrocytes, and then the biologic characters of the chondrocyte were observed and tested.
方法体外培养获取生长状态良好的兔骨髓间充质干细胞，进行成软骨诱导，观察并检测其生物学特性。
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