• Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.

    实时荧光定量PCR技术利用荧光检测方法定量核酸技术,具有高度灵敏性特异性精确性

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  • Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.

    目的建立诺如病毒荧光定量PCR检测方法,应用急性胃肠炎快速检测

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  • Methods:Viral RNA Was extracted from feces. 92 samples were detected by real-time fluorescent quantitative PCR and Enzyme-linked Immunosorbent Assay(ELISA).

    方法提取粪便中的RNA实时荧光PCR方法对丽水市急性胃肠炎的92份标本进行检测与常规ELISA检测结果比较。

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  • Article on the principle of real-time fluorescent quantitative PCR, factors and plant diseases and genetic breeding of the application were reviewed.

    文章实时荧光定量pcr原理影响因素以及植物病害遗传育种方面应用进行了综述。

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  • To establish a TaqMan-based real-time fluorescent quantitative PCR assay for detection and quantitation of Hepatitis E virus.

    目的建立灵敏、稳定、特异的实时荧光PCR方法,用于戊型肝炎病毒的定量检测

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  • Results:Norovirus was successfully detected from epidemic sample of Lishui, the positive rates of real-time fluorescent quantitative PCR and ELISA were 78.26% and 55.43%, respectively.

    结果:用实时荧光PCR方法成功丽水疫情样本检测出诺如病毒,实时荧光PCRELISA的检出率分别为78.26%和55.43%。

    youdao

  • Results:Norovirus was successfully detected from epidemic sample of Lishui, the positive rates of real-time fluorescent quantitative PCR and ELISA were 78.26% and 55.43%, respectively.

    结果:用实时荧光PCR方法成功丽水疫情样本检测出诺如病毒,实时荧光PCRELISA的检出率分别为78.26%和55.43%。

    youdao

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