Using fluorescence quantitative PCR method of quantitative analysis.
采用荧光定量pcr的方法进行定量分析。
The correspondence of the HCMV pp65 test and quantitative PCR was 92.3%.
免疫荧光法和实时定量PCR有较好的一致性（92.3%）。
Differentially expressed genes were identified by real-time quantitative PCR.
采用实时荧光定量PCR鉴定高表达基因。
Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
结果建立的实时荧光定量PCR方法的灵敏度为10-4水平。
Real time quantitative PCR has been widely used in various areas of plant pathology research.
实时荧光定量pcr广泛应用于植物病理学研究的各个领域。
Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs in China.
方法采用半定量pcr方法检测了我国四种小型猪内源性逆转录病毒基因的拷贝数。
Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis.
目的探讨荧光定量聚合酶链反应（FQ-PCR）技术诊断结核病临床应用价值。
The real time quantitative PCR is applied to research the expression trend of these genes under five different hormones.
并通过荧光定量PCR研究其在外施五种激素的条件下的胶乳中的表达谱。
To establish a TaqMan-based real-time fluorescent quantitative PCR assay for detection and quantitation of Hepatitis E virus.
目的建立灵敏、稳定、特异的实时荧光PCR方法，用于戊型肝炎病毒的定量检测。
The copies of IS1112 in twelve Xoo strains from China were identified using realtime quantitative PCR with this standard plasmid.
利用该标准质粒建立的定量方法测定了我国12种白叶枯菌株基因组中IS1112的拷贝数。
Objective To investigate the clinical application of the Fluorescence quantitative PCR (FQ-PCR) in the detection of HCV infection.
目的：用荧光定量聚合酶链反应(FQ - PCR)测定丙型肝炎病毒在临床的应用。
Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.
目的：建立诺如病毒的荧光定量PCR检测方法，应用于急性胃肠炎的快速检测。
Objective: to develop an internal quality control substance of HBV-DNA by real time quantitative PCR and to evaluate its clinical value.
目的：制备h BV - DNA荧光定量pcr检测的室内质控物，并对其应用价值进行初步评价。
Conclusion a higher level of consistency and repeatability was found from the method of semi-quantitative PCR-RFLP compared to PCR-RFLP.
结论应用半定量pcr方法可明显提高P CR -RFLP酶切法的精确性和可重复性。
Then Semi-quantitative PCR was performed with 27 cycles and the result was that adiponectin mRNAs in lean pigs were higher than obese one.
以27为最终循环数进行半定量PCR，结果显示瘦肉型猪的表达量高于脂肪型。
Using this model to do quantitative PCR analysis, result error is only related to the accuracy of fluorescence intensity or the instrument used.
使用动力学数学模型做定量pcr分析，其结果的误差仅与使用的荧光强度数值的精确度相关。
The tissue samples from pigs were detected by using the established quantitative PCR assay, and the results was compared with that of routine PCR.
用建立的检测方法对临床采集的组织病料进行了检测，并与常规PCR作对比。
Cytology on BKV infection was carried out and was compared with PCR. Cytology has less sensitivity and specificity than real-time quantitative PCR.
开展了BK病毒感染细胞学研究，并与PCR方法作了比较，认为细胞学检查灵敏度和特异度均低于定量pcr法。
Article on the principle of real-time fluorescent quantitative PCR, factors and plant diseases and genetic breeding of the application were reviewed.
文章对实时荧光定量pcr的原理、影响因素以及在植物病害和遗传育种方面的应用等进行了综述。
The engraftment state of the donor cells into recipients was confirmed by microsatellite DNA fingerprinting and fluorescent quantitative PCR analysis.
移植后供者细胞植入状态的检测方法采用微卫星DNA指纹法或萤光定量pcr分析。
Methods The serum samples from 310 cases of viral hepatitis were tested by Fluorescence quantitative PCR assay (FQ PCR), and also by ELISA as contrast.
方法运用荧光定量pcr (FQ - pcr)和el IS A两种方法同时检测了310份肝炎患者血清，并对结果进行了对比分析。
In addition, the differences of these genes were studied by real-time quantitative PCR which in two soybean lines and different seed developmental stages.
此外，还利用荧光定量pcr技术进一步分析了这六个基因在两个大豆品种中的定量差异。
Methods:Viral RNA Was extracted from feces. 92 samples were detected by real-time fluorescent quantitative PCR and Enzyme-linked Immunosorbent Assay(ELISA).
方法：提取粪便中的RNA，用实时荧光PCR方法对丽水市急性胃肠炎的92份标本进行检测，并与常规ELISA检测结果比较。
The principles and results of laboratory detecting techniques of SARS such as immunological assay, quantitative PCR and biochip are introduced in the thesis.
本文对目前已经建立的SARS实验室检测技术(如免疫学、定量pcr和生物芯片技术)和原理以及实际应用结果进行了介绍。
To filter out some of the differential genes, 18 cases of AS group and 6cases of healthy control group are validated by real-time fluorescence quantitative PCR.
对筛选出的部分差异基因，选取病例组18例及正常对照组6例，以实时荧光定量PCR进行验证。
Methods The expression of TUSC3 gene in 20 cases of pancreatic cancer and 6 cases of normal pancreas were detected by oligonucleotide microarray and real-time quantitative PCR.
方法利用寡核苷酸基因芯片技术和实时定量pcr技术检测20例胰腺癌组织和6例正常胰腺组织中TUSC3基因的表达。
This research established a sensitive quantitative PCR method for SIV load testing. The creteria for accuracy judgement of duplication in quantitative PCR method was introduced.
本研究建立了敏感的SIV定量PCR检测方法，并提出了判断复孔检测结果是否准确的计算方法。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Results:Norovirus was successfully detected from epidemic sample of Lishui, the positive rates of real-time fluorescent quantitative PCR and ELISA were 78.26% and 55.43%, respectively.
结果：用实时荧光PCR方法成功的从丽水疫情样本中检测出诺如病毒，实时荧光PCR和ELISA的检出率分别为78.26%和55.43%。
Results:Norovirus was successfully detected from epidemic sample of Lishui, the positive rates of real-time fluorescent quantitative PCR and ELISA were 78.26% and 55.43%, respectively.
结果：用实时荧光PCR方法成功的从丽水疫情样本中检测出诺如病毒，实时荧光PCR和ELISA的检出率分别为78.26%和55.43%。
应用推荐