• Conclusion the FV quantitative assay was validated for the diagnosis and classification of FV deficiency.

    结论该法是一种较好的FV蛋白定量测定法,可以对FV缺乏症进行辅助诊断和分型。

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  • In the second part of the study, A quantitative assay for JAK2V617F mutation in 136 CMPN patients by ARMS-PCR and capillary electrophoresis.

    通过毛细管电泳检测患者JAK2V617F突变转录本水平,定量分析JAK2V617F突变与部分临床参数间的相关性。

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  • Objectives: to Research the effects of different calibration ways of biochemistry quantitative assay on the results and the tolerance of the differences.

    目的:探讨常规生化定量测定法中应用多点校准和单点校准模式校正分析仪,对测定结果的影响并评价其差异的可接受性。

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  • We have established a rapid and sensitive method-ELISA for quantitative assay of 64DP. 24 samples were parellelly examined by ELISA and rocket electrophoresis.

    本文采用亲和层析法纯化的兔抗64DP抗体,建立了简便灵敏的酶免疫测定法(ELISA)。

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  • The optimized MTT method was a rapid, simple, convenient, and sensitive quantitative assay to evaluate the viability of Candida albicans and other yeast or yeast- like fungi.

    结果提示优化的MTT方法可以快速、简便、灵敏和定量地分析白念珠菌及某些致病性酵母菌和酵母样真菌的活力。

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  • Methods: According to turbidimetric assay, quantitative analysis of bacterial endotoxin which infusion contained.

    方法:采用动态浊度法,定量分析输液中所含细菌内毒素。

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  • The adherence of 19 SE strains isolated from patients was assayed by three different methods, namely, quantitative adherence assay, scanning electron microscopy (SEM) and light microscopy.

    用半定量粘附实验、扫描电子显微镜法和光学显微镜法检测自临床分离到的19株表葡的粘附能力。

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  • The plasma endotoxin levels of 91 hepatopaths and 20 healthy controls were detected by using a quantitative endotoxin assay of limulus amoebocyte lysate (LAL) test with a chromogenic substrate.

    本文采用鲎变形细胞溶解物(LAL)改良基质显色法对91例肝脏病患者和20例健康对照血浆内毒素进行定量检测。

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  • Objective To develop a semi-quantitative gold immunofiltration assay(GIFA) with quality control dots for the detection of alpha-fetoprotein(AFP) in serum.

    目的建立一种伴有质控点的金免疫渗滤法(GIFA),用于半定量检测血清甲胎蛋白(AFP)。

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  • Methods The serum samples from 310 cases of viral hepatitis were tested by Fluorescence quantitative PCR assay (FQ PCR), and also by ELISA as contrast.

    方法运用荧光定量pcr (FQ - pcr)和el IS A两种方法同时检测了310份肝炎患者血清,并对结果进行了对比分析。

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  • Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.

    目的:建立诺如病毒的荧光定量PCR检测方法,应用于急性胃肠炎的快速检测。

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  • Objective To introduce a quantitative colorimetric assay based on a tetrazolium salt (MTT).

    目的介绍一种用四氮唑蓝(MTT)为底物的酶反应定量比色分析法。

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  • Objective To set up a duplex quantitative real-time PCR (QPCR) assay with high sensitivity, specificity and rapidity to detect Candida krusei and Candida glabrata.

    目的建立一种快速、灵敏、特异的鉴定克柔念珠菌和光滑念珠菌的双重实时荧光定量PCR方法。

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  • The principles and results of laboratory detecting techniques of SARS such as immunological assay, quantitative PCR and biochip are introduced in the thesis.

    本文对目前已经建立的SARS实验室检测技术(如免疫学、定量pcr和生物芯片技术)和原理以及实际应用结果进行了介绍。

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  • Methods:Viral RNA Was extracted from feces. 92 samples were detected by real-time fluorescent quantitative PCR and Enzyme-linked Immunosorbent Assay(ELISA).

    方法:提取粪便中的RNA,用实时荧光PCR方法对丽水市急性胃肠炎的92份标本进行检测,并与常规ELISA检测结果比较。

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  • Each quantitative analytical procedure should be designed to minimize assay variation.

    定量分析方法时都应当要减少其分析误差。

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  • The tissue samples from pigs were detected by using the established quantitative PCR assay, and the results was compared with that of routine PCR.

    用建立的检测方法对临床采集的组织病料进行了检测,并与常规PCR作对比。

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  • Methods Hepatitis B serum markers and Pre-S2 antigens were tested by enzyme-linked immunosorbent assay and HBV DNA was detected by fluorescent quantitative-PCR in 982 hepatitis B patients.

    方法用酶联免疫吸附试验对982例乙肝患者血清标志物和乙肝病毒前S2抗原进行检测;并用荧光定量PCR法对其进行HBV-DNA检测。

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  • To establish a TaqMan-based real-time fluorescent quantitative PCR assay for detection and quantitation of Hepatitis E virus.

    目的建立灵敏、稳定、特异的实时荧光PCR方法,用于戊型肝炎病毒的定量检测。

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  • This assay employs the quantitative sandwich enzyme immunoassay technique. Platelet antigens have been pre-coated onto a microplate.

    本试剂盒应用双抗体夹心酶标免疫分析法测定标本中血小板抗体水平。

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  • This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for lymphocyte factor has been pre-coated onto a microplate.

    本试剂盒应用双抗体夹心酶标免疫分析法测定标本中淋巴细胞因子水平。

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  • This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for lymphocyte factor has been pre-coated onto a microplate.

    本试剂盒应用双抗体夹心酶标免疫分析法测定标本中淋巴细胞因子水平。

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