AIM: Three-dimensional cultured model of pulp cell was established to provide a tool in the research of pulp disease.
目的：建立人牙髓成纤维细胞体外三维立体培养模型，为研究牙髓生理病理奠定基础。
The mechanism of apoptosis is not clear right now. Further study of dental pulp cell apoptosis will have a positive impact on dental pulp physiopathology.
关于牙髓程序性细胞死亡的相关研究不多，机制也尚不清楚，因此对其进行研究将会对牙髓的生理病理学产生深远的意义。
Conclusion the expression of TIMP-1, -2 in human dental pulp cell and tissue is detected, and this provides the basis for further studying on the mechanism of TIMP in dental pulp.
结论TIMP 1、2在人牙髓成纤维细胞及牙髓组织均有表达，为进一步研究TIMP在牙髓组织中的作用机理提供实验依据。
CONCLUSION: the cytotoxic effect of 4 kinds of dental restorative materials on pulp cell was relatively low in vitro, which indicated that all the materials has good biocompatibility.
结论：4种牙体修复材料均对牙髓细胞的生长无不良影响，具有良好的生物相容性。
Methods:The culture supernatant of monocytes stimulated with Fn LPS was applied to human dental pulp cell in vitro, and activity of ALP in human dental pulp fibroblast was monitored by OD test.
方法：采用经具核梭杆菌LPS刺激的单核细胞培养上清作用于体外培养的人牙髓细胞，通过OD值测定，观察单核细胞培养上清对人牙髓成纤维细胞碱性磷酸酶（ALP）活性的影响。
Methods:The culture supernatant of monocytes stimulated with Fn LPS was applied to human dental pulp cell in vitro, and activity of ALP in human dental pulp fibroblast was monitored by OD test.
方法：采用经具核梭杆菌LPS刺激的单核细胞培养上清作用于体外培养的人牙髓细胞，通过OD值测定，观察单核细胞培养上清对人牙髓成纤维细胞碱性磷酸酶（ALP）活性的影响。
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