Some scholars have established a PCR for testing PRRSV, PCV2 and CSFV, but the multiplex PCR for the three viruses is none.
有学者建立了检测prrsv、PCV2和CSFV的PCR技术，但同时检测这三种病毒的多重pcr方法还未见报道。
In thymus, the PRRSV positive cells most often were seen in the medulla, but few positive cells were present in the cortex.
胸腺中PRRS病毒多出现在髓质部，皮质中有少许细胞呈阳性反应。
RT PCR test proved that the isolate belongs to serotype of American PRRSV and shares the same subgenotype with ATCC VR2332.
结合病毒分离鉴定确诊为PRRS病毒（PRRSV）感染，RTPCR试验证实分离毒属美洲型PRRSV ，且与ATCC VR2 332株属同一基因亚型。
With the statistical analysis of biological, at present, the variant infections of PRRSV is overwhelming in Anhui province.
生物统计结果表明，当前安徽省猪群中PRRSV的感染以变异株为主。
A new method for titration of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) was developed utilizing immunostaining.
本文建立一种测定猪繁殖与呼吸综合征病毒(PRRSV)滴度的新方法-免疫染色法。
For its infectiousness as well as the immune repression and intercurrent brought to pigs, PRRSV caused huge economic loss to pig's feeding industry.
该病毒传染性强，而且感染机体后导致免疫抑制及各种并发症，给养猪业造成巨大危害和经济损失。
The aetiological study has isolated and identified PRRSV and PRV for the first time in Gansu, it confirmed the existence of the above two diseases in Gansu.
通过病原学研究，在甘肃省首次分离并鉴定了PRRSV和PRV，确诊了以上两病在甘肃省规模化养猪场中的存在。
PRRSV entered Marc-145 cells by endocytosis, and replicated in the cytoplasm. The mature viruses were released from infected cells by budding or exocytosis.
病毒感染细胞后以细胞内吞方式进入细胞，在胞浆内复制，装配好的病毒以出芽或细胞外分泌释放到细胞外。
The effect of mucosal immune of porcine reproductive and respiratory syndrome virus (PRRSV) attenuated and disactived vaccine was investigated by histological method.
应用组织学方法比较观察了猪繁殖与呼吸综合征病毒（PRRSV）弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫的效果。
The result showed that PRRSV can be quickly diagnosed by RT PCR, which laid a basis for quick diagnosis of PRRS and further research of the PRRSV isolates of our country.
结果说明应用所设计的引物进行RTPCR快速检测PRRS是可行的，为我国快速特异诊断PRRS和PRRSV强毒株的深入研究奠定了基础。
The MAbs against PRRSV GP5 can be used for further analysis of the structure and function of PRRSV, identification of epitopes on GP5, which will be helpful for new vaccine design.
本研究获得的单克隆抗体将为今后深入研究PRRSV病毒结构和功能，分析GP5蛋白的抗原表位，设计有效的新型疫苗等提供有益帮助。
A kit for detection of antibody against porcine reproductive and respiratory syndrome virus (PRRSV) was prepared by immunoperoxidase monolayer assay (IPMA) and assembled as PRRSV-IPMA kit.
建立了一种检测猪生殖与呼吸综合征病毒(PRRSV)血清抗体的免疫过氧化物酶单层细胞试验法(IPMA)，并组装成PRRSV - IPMA抗体检测试剂盒。
Three currently available commercial PCV2 vaccines were compared in the conventional pig model using pigs with passively acquired anti-PCV2 antibodies with or without experimental PRRSV infection.
现有三款商业PCV2病毒疫苗进行了比较传统的养猪模式采用被动获得抗pcv2抗体或没有实验prrsv感染猪。
Infection of PRRSV can not only damage the immunity of the swine but also cause secondary affection or complicating disease involved other pathogenic microorganism, which exacerbates the situation.
PRRSV感染能降低猪体免疫力，常引起其他病原微生物的继发或并发感染，加重病情。
Using recombinant nucleocapsid protein GST-N of porcine reproductive and respiratory syndrome virus (PRRSV) as antigen, an indirect ELISA for the detection of antibodies against PRRSV was developed.
用亲和层析纯化的猪繁殖与呼吸综合征病毒(PRRSV)重组核衣壳蛋白gst - N作为诊断抗原，建立检测prrs血清抗体的间接ELISA方法。
The result shows this method can be used in the diagnosis of PRRSV infection and the research for PRRSV distribution in the infected tissues, and the retrospective diagnosis in formalin-fixed tissues.
该法可用于仔猪PRRSV感染的诊断和组织中核酸的定位及分布研究，也可用于甲醛固定组织的回顾性诊断。
The result shows this method can be used in the diagnosis of PRRSV infection and the research for PRRSV distribution in the infected tissues, and the retrospective diagnosis in formalin-fixed tissues.
该法可用于仔猪PRRSV感染的诊断和组织中核酸的定位及分布研究，也可用于甲醛固定组织的回顾性诊断。
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