Cortical microtubules were observed in intraepidermal cells of onion bulb with a thin section and negative staining of protoplasts.
洋葱鳞茎表皮细胞内可见皮层微管，呈薄片状，原生质体染色阴性。
Transformation of HGE protoplasts and screening of the mutants.
禾长蠕孢菌原生质体转化及突变体筛选与验证。
The fusion rate of the inactivated parental protoplasts was 3.31%.
所得双亲菌株原生质体的融合率为3.31%。
The protoplasts of attached cells are connected and integrated by plasmodesmata.
接触的细胞的原生质体通过原生质连丝连接成一体。
There was also no significant difference in the yield of protoplasts from different strains.
不同菌株原生质体产量差异不显著。
The colonies grew up from the fusants or protoplasts plated on solid medium after electrofusion.
电融合后的融合子和原生质体在固体培养基上植板培养成菌落。
The mesophyll cell protoplasts of potato could divide from cell colonies and callus after cultured.
马铃薯叶肉细胞原生质体培养后，再生细胞形成细胞团和愈伤组织。
Yieldand viability of mesophyll protoplasts were increased as Cellulase concentration was increased.
随纤维素酶浓度升高，原生质体产量及活力增大。
High yield of protoplasts has been obtained by using enzyme containing 1% snailase or 1.5% lywallzyme.
蜗牛酶和1.5％溶壁酶酶解金针菇菌丝获得了高产量的原生质体。
So it is a key step to establish an effective system of preparation and regeneration of HGE protoplasts.
因而，原生质体制备和再生技术是禾长蠕孢菌转化与基因克隆技术的基础。
Protoplasts were cultured in KPR medium with 0. 6% agarose. The plating efficiency of protoplasts was 12. 0%.
纯化后的原生质体在KPR培养基中进行琼脂糖包埋培养，原生质体植板率为12.0%。
The isolated protoplasts whose sizes were plumpness and round in size and vigor were nice determined with FDA.
分离出的原生质体形态饱满浑圆，FDA测定活力良好。
It was found that the cytoplasm of the protoplasts were rich in very dense and fine actin microfilament cables.
研究结果发现洋葱鳞茎内部细胞的细胞质内存在极丰富而精细的肌动蛋白微丝束。
The rates of formation and regeneration of protoplasts of strain S23 had reached to 93.8% and 37.5% respectively.
稳定剂为琥珀酸钠，再生培养方法为平板双层法。原生质体形成率和再生率分别达到93.8%和37.5%。
A total number of 206 regenerated plants (R2) of 6 plant lines of rice strain 77-170 from protoplasts were obtained.
获得了粳型水稻77- 170品系原生质体再生植株(R_2)6个株系的206棵后代植株。
Intact cells were found to be more resistant to the detrimental effects of electric field than that of the protoplasts.
与原生质体比较，完整细胞可以耐受更高幅值的电脉冲。
Cortical microtubules were observed in intraepidermal cells of onion bulb with thin section and negative staining of protoplasts.
用超薄切片和原生质体负染的方法，在电镜下观察到洋葱鳞茎内表皮细胞周质微管的存在。
Results from different treatments showed that the pollen tube growing phenomenon was from the fusion between two types of protoplasts.
不同处理的结果显示，这一现象来源于异核体细胞。
The yield of Gibberellin and morphology of colonies of the regenerated strains from protoplasts are the same as those of the parent strain.
原生质体再生菌株仍保留亲株的菌落形态及合成赤霉素的生产能力。
This study would be of value for establishing high-effective regeneration system of protoplasts and the related genetic manipulations in longan.
研究结果对建立高效的龙眼原生质体再生系统及其遗传操作有参考价值。
Under the optimum conditions, the rates of formation and regeneration of protoplasts of stain SD-142 had reached to 86.8% and 31.3% respectively.
在原生质体形成及再生的最佳条件下制备原生质体，结果发现，SD- 142菌的原生质体形成率及再生率分别达到了86.8%和31.3%。
The preparation of monokaryon protoplasts and the screening of protoplast-derived monokaryon strains are important parts in mushroom research and breeding.
研究了利用珍稀食用菌茶树菇双核菌丝进行原生质体分离和再生，以及原生质体单核菌株的筛选过程。
Inactivated protoplasts fusion method has more advantages in breeding techniques and also it has been used effectively in parents without any heritable labels.
双亲灭活原生质体融合技术优点较多，是原生质体融合育种中针对无遗传标记同种不同株双亲的一种常用的有效方式。
This paper elaborates the discovery and effect of monokaryon and homokaryon protoplasts and their application in genetic and breeding research of edible mushroom.
本文阐述了食用菌单核和同核原生质体的发现和作用以及在食用菌遗传研究上的应用前景。
After Genetic background analysis of Streptomyces diastaticus No. 7 strain M1033, the modified conditions of M1033 protoplasts and transformation were established.
对7号淀粉酶菌M1033的遗传背景进行分析，建立了其原生质体制备、转化的优化条件。
The results promote the studies of isolating somatic cells and protoplasts as well as the popularization of indoor artificial breeding technology of somatic cells .
研究结果为海藻细胞及原生质体分离研究及海藻体细胞育苗的推广应用打下了良好的基础。
Ordinary digital cameras are unable to manufacture zoom pictures of protoplasts by means of traditional (film) photography methods because its lens cant be disassembled.
普通数码相机，由于其镜头不能拆卸，因此，无法按传统(胶片)的照相方法进行原物大照相，制作原物大照片。
The results showed that after 24 h in culture a number of protruding vesicles, as well as a small amount of fibrillar component were formed on the surface of protoplasts.
结果表明，离体培养24小时的原生质体表面产生一些突起小泡，有时可见少量纤维组分的形成。
The experiment has proved that TMV-RNA multiplied vigorously in tobacco protoplasts with cellular nucleuses, while hardly multiplied in cytoplasts separated from nucleuses.
本试验证明了TMV - RNA在有细胞核的烟草原生质体中能大量增殖，但在无核的胞质体中却难以增殖。
The direct toxic effects of oxalic acid on detached leaves, calli, isolated cells and protoplasts of oilseed rape (Brassica napus) were determined at organ and cell levels.
研究了油菜菌核病草酸毒素在组织和细胞水平的毒性影响。
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