Low level of sucrose is required during induction of Protocorm Like-Body and shoots differentiation.
类原球茎诱导分化过程中所需的蔗糖浓度相对较低。
The growth curve of Dentrobium cell showed "s" type when the protocorm were cultured on solid media or with liquid suspension way.
固体培养和液体悬浮培养的石斛原球茎细胞生长曲线大致呈“S”型。
Effects of basal culture medium, sucrose concentration, natural extracts and phytohormones on protocorm differentiation were studied.
试验了基本培养基、蔗糖浓度、天然提取物以及植物激素对铁皮石斛原球茎分化的影响。
There were many different reports on the multiplication and differentiation of protocorm and the choice of culture media, but they need further test and verify in practice.
原球茎的增殖、分化与培养基的选择有不同的结论，需要作进一步验证。
Objective: To study the relationship between growth of Protocorm-like bodies and accumulation of total alkaloids and polysaccharides (two active constituents) in Dendrobium moniliforme.
目的：研究细茎石斛拟原球茎生长规律及其与两种有效成分总生物碱和可溶性多糖积累的关系。
Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants, N6 was used as the basic culture with phytohormone added, and fungal extracts as the elicitor.
取继代培养30 d的铁皮石斛类原球茎作接种材料，以N6为基本培养基，加入植物激素，并以真菌提取物作诱导子，对类原球茎和愈伤组织进行诱导培养。
Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants, N6 was used as the basic culture with phytohormone added, and fungal extracts as the elicitor.
取继代培养30 d的铁皮石斛类原球茎作接种材料，以N6为基本培养基，加入植物激素，并以真菌提取物作诱导子，对类原球茎和愈伤组织进行诱导培养。
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