Primer design is the key to our success, the primer sequences have designed specifically according to the reference.
引物的设计很关键，特异的引物序列设计参照文献。
W4 and W6 derived from single primer amplification, and had no homology to the known sequences.
W4和W6是单引物扩增产物，与已知序列无同源性。
Single primer PCR was a method of chromosome walking to isolate sequences flanking a known DNA sequence with only one primer.
单引物pcr是一种只用一条引物就可以克隆已知序列侧翼未知区域的染色体步移方法。
ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
通过引物设计、P CR、基因克隆、测序，获得了10种鸢尾属植物和1个外类群种的ITS序列。
ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
通过引物设计、P CR、基因克隆、测序，获得了10种鸢尾属植物和1个外类群种的ITS序列。
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