NECL2 distributed in cell body and protuberance of primary culture neuron. The rat adrenal gland tumor cell line PC12 can be induced into neuronal differentiation.
大鼠肾上腺嗜铬细胞瘤细胞PC12具有神经元样分化的潜能，在PC12细胞中转染并过量表达NECL2能够诱导细胞发生神经元样分化。
Objective: To establish an easy and high-yield method of rat dopaminergic neuron primary culture, and to observe the damage to the neurites by the trypsogen.
目的：建立一种简单高效的中脑多巴胺神经元细胞原代培养方法，并观察胰酶消化对中脑多巴胺能神经元突起生长的损伤作用。
Objective the aim was to observe the effect of lead on the activities of ERK in plasma of rat primary hippocampal neuron culture.
目的观察不同浓度和不同时间染铅对大鼠海马神经元培养细胞细胞质erk活性的影响。
Methods: Primary embryonic rat cortical neurons culture, cytotoxicity assays and biochemistry detection, cell stain for the neuron morphology in microscopy and image analysis.
方法：原代胚胎鼠皮质神经元细胞培养，细胞毒性试验及生化指标测定，细胞染色，显微镜下观察形态学改变及影像分析。
Methods: Primary embryonic rat cortical neurons culture, cytotoxicity assays and biochemistry detection, cell stain for the neuron morphology in microscopy and image analysis.
方法：原代胚胎鼠皮质神经元细胞培养，细胞毒性试验及生化指标测定，细胞染色，显微镜下观察形态学改变及影像分析。
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