AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
Rifampicin cannot bind to the mutant RNA polymerase and so is unable to block the initiation of transcription.
利福平不能结合到突变的RNA聚合酶上，因此不能阻断转录的起始。
Rifampicin cannot bind to the mutant RNA polymerase and so is unable to block the initiation of transcription.
利福平不能结合到突变的RNA聚合酶上，因此不能阻断转录的起始。
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