A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee (Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.
基于中蜂囊状幼虫病病毒基因组序列AF469603中的RNA依赖的RNA聚合酶基因，建立该病毒的半套式RT-PCR检测方法。
Eligible patients were hepatitis B surface antigen-positive men and women with compensated liver disease who were given lamivudine at least more than 6 months and had HBV polymerase gene mutation.
符合条件的患者乙肝表面抗原阳性的代偿性肝脏疾病的男性和女性谁是至少6个月以上的，给予拉米夫定和HBV聚合酶基因突变。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
酚-氯仿法从外周血中提取基因组dna，聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
For example, RNA polymerase II is found concentrated at the promoters of thousands of genes, causing much interest in the field to shift focus to the role of elongation in regulating gene expression.
例如，RNA聚合酶ii发现集中在成千上万个基因的促销员，导致在该领域的极大兴趣，在调节基因表达的重点转移到伸长的作用。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
A 40-base polymorphic repeat sequence located in the 3 '-untranslated region of the DAT gene was purified and amplified by polymerase chain reaction (PCR).
将位于多巴胺运输器基因3'端未转译区段的40 -碱基多形性重复序列予以纯化、经聚合酶链锁反应放大。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
Polymerase chain reaction (PCR) technology has been successfully used in clinic diagnostic and modern molecular biology for gene analyzing.
聚合酶链式反应(PCR)技术已在分子生物学、基因测序、医学诊断等方面得到广泛的应用。
Methods Polymerase chain reaction(PCR)was used for 12 JAK2V617F -negative PV patients to amply the region of JAK2 exon 12, direct gene sequencing was performed to detect mutations of JAK2 exon 12.
方法采用聚合酶链反应（PCR）扩增12例JAK2V617F点突变阴性PV患者的JAK2外显子12片段，经基因测序与野生型JAK2外显子12比对，了解是否存在JAK2外显子12突变。
Through DNA polymerase reaction, a modified lipase gene was obtained.
通过DNA聚合酶反应，获得了改造的荧光假单孢菌脂肪酶基因。
Suddenly, RNA polymerase is let go, raising along the DNA to read the gene.
RNA聚合酶突然被启动，沿着DNA上升并读取基因。
The gene expression levels for interstitial collagenase and tissue inhibitor of metallo-proteinase-1 (TIMP-1) were measured by reverse transcription polymerase chain reaction.
逆转录多聚酶链反应检测间质胶原酶及金属蛋白酶组织抑制因子- 1基因表达水平。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
采用单链聚合酶链反应(PCR)和测序法检测肿瘤组织中VHL基因的突变情况。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Gene of polyketide synthase (PKS) from 2 toxin-producing cyanobacteria was prepared by polymerase chain reaction and the gene sequence was analyzed.
应用聚合酶链反应得到2株蓝绿藻的毒素聚酮合成酶(PKS)基因，并进行基因序列分析。
Methods 40 patients with lung cancer were studied to detect TTF-1 gene by using reverse transcription-polymerase chain reaction assay (RT-PCR).
方法采用逆转录—聚合酶链反应(RT -PCR)方法检测40例患者的肺癌组织ttf - 1基因的表达。
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of active efflux gene CDR1 and CDR2.
用逆转录-聚合酶链反应(RT - PCR)方法检测主动外排泵基因CDR1和CDR2的表达水平。
Extron 1-5 of RHO gene was amplified by polymerase chain reaction (PCR), and the mutation of RHO gene was screened by direct DNA sequence measurement.
采用聚合酶链反应(PCR)方法扩增rho基因第1 ~ 5外显子和第1内含子基因片段，用直接dna测序法筛查rho基因突变。
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
方法运用序列特异性引物聚合酶链反应技术，检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
Method all exons of ATP2C1 gene were analyzed with polymerase chain reaction and DNA sequencing in all patients of this family and 100 unrelated population-match controls.
方法采用聚合酶链反应扩增该家系患者和健康对照个体atp2c1基因的全部外显子，直接测序法进行DNA测序，100例无亲缘关系的正常人作为对照。
The gene expression of ET-1, ETAR, ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response (RT-PCR).
采用半定量逆转录多聚酶链反应(RT - PCR)检测局部内皮素系统ET - 1、ETAR、ETBR及ECE的基因表达。
G1 gene sequence of m fragment from hantavirus genome was amplified by reverse transcription polymerase chain reaction (RT-PCR) and analyzed.
采用逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒基因组M片段G1区基因序列并测序。
We constructed a new type gene encoding human TNP-o by using polymerase chain reaction CPCR technique. The gene was highly expressed in e. coli.
在TNP结构与功能关系研究的基础上，该文作者利用PCR技术构建了一种新型人tnf的编码基因，并使其在大肠杆菌中进行了高效表达。
We constructed a new type gene encoding human TNP-o by using polymerase chain reaction CPCR technique. The gene was highly expressed in e. coli.
在TNP结构与功能关系研究的基础上，该文作者利用PCR技术构建了一种新型人tnf的编码基因，并使其在大肠杆菌中进行了高效表达。
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