Using polyamide column chromatography, gathering effluent fraction of ethanol, applying rutin as standard curve, we estimated the content of crude flavonoids.
乌蕨提取物经过聚酰胺柱层析，收集乙醇洗脱部分，以芦丁作标准曲线，用比色法测定总黄酮。
The ethyl acetate and n-butanol extraction were chromatographed on silica gel column, polyamide column, sephadex LH-20 column and preparing TLC, respectively.
采用硅胶、聚酰胺、葡聚糖凝胶LH-20柱层析法和制备薄层层析法对乙酸乙酯和正丁醇提取物进行了分离、纯化。
Method:To establish the most appropriate method by comparing the content of Narrigin in sample which did not pass polyamide column and passed various granule grades polyamide columns.
方法：比较样品提取中不过聚酰胺柱以及过不同粒度的聚酰胺柱的柚皮苷的含量，以确定最合适的方法。
Method:To establish the most appropriate method by comparing the content of Narrigin in sample which did not pass polyamide column and passed various granule grades polyamide columns.
方法：比较样品提取中不过聚酰胺柱以及过不同粒度的聚酰胺柱的柚皮苷的含量，以确定最合适的方法。
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