Results polyadenylation signal-deficient retroviruses could be packaged by PA317 packaging cells.
结果多聚腺苷酸化信号缺陷病毒可以在PA317病毒包装细胞形成病毒颗粒；
The ability to make mRNAs of varying sizes from one coding region, by altering the site of pre-mRNA cleavage and polyadenylation.
通过改变前体mrna的切割位点和聚腺苷酸化位点而从一个编码区产生不同大小mrna的能力。
The underlined nucleotides are the first upstream in-frame stop codon and the two putative polyadenylation signals, respectively. The asterisk indicates the stop codon.
加下划线的核苷酸分别是这个读码框中上游的第一个终止密码和两个已经认定的多核苷酸序列。星号标记的就是终止密码。
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
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