Objective: To develop an efficient economical and environmental method for the preparation of highly purified plasmid DNA.
目的：建立简便高效、成本低廉和安全无污染的高纯度质粒提取方法。
It was therefore preparation for constructing an expression plasmid vector to express exogenous genes.
为构建其启动外源基因的质粒表达载体作准备。
Objective To explore a procedure for preparation of plasmid DNA for gene therapy.
目的探索基因治疗用质粒DNA的制备工艺。
The preparation process of the immunotoxin plasmid includes the construction of the recombinant plasmid, the preparation of the recombinant transinfected engineering bacterium and other steps.
该免疫毒素质粒的制备方法包括重组质粒的构建、重组质粒转染的工程菌的制备等步骤。
Results and Conclusion: the PCR screening method was convenient and fast for confirming positive recombinant clone, and there was no need for preparation and purification of the plasmid.
结果和结论：以pcr方法筛查重组阳性克隆，可以简便快速鉴定重组阳性克隆，不需提取质粒。
Results and Conclusion: the PCR screening method was convenient and fast for confirming positive recombinant clone, and there was no need for preparation and purification of the plasmid.
结果和结论：以pcr方法筛查重组阳性克隆，可以简便快速鉴定重组阳性克隆，不需提取质粒。
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