In this review, the application of anion exchange chromatography to pharmaceutical plasmid analysis was reviewed.
本文对阴离子交换色谱在药用质粒分析方面的应用与研究进展进行综述。
The results showed that plasmid analysis, in particular with outer membrane protein profiles were useful methods in epidemiological investigation of nosocomial infections due to Acinetobacter.
结果表明：质粒分析、尤其是结合外膜蛋白图谱分型可较好地用于不动杆菌医院感染的流行病学调查。
RAPD analysis had high distinguish ability and could provide more direct, rich and precise genetic information than plasmid profile analysis.
相对于质粒分析而言，RAPD分析具有更高的分辨率，它提供的遗传学信息更为丰富、直接、精细。
Plasmid DNA profiles analysis offer scientific reference to molecular epidemiology investigation of avian colibacillosis .
质粒DNA图谱分析为从分子水平上进行鸡大肠杆菌病流行病学调查提供了基础材料。
Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment.
限制性酶切图谱分析证实这两个基因的结构是完整的，符合转基因实验要求。
RESULTS: DDR2/EGFP fusion plasmid was successfully constructed. Further analysis also demonstrated that the fusion protein has similar expression and activation pattern with wild type ones.
结果：成功构建了DDR2/EGFP融合表达载体，进一步的分析也证明此融合表达载体能在细胞中正确表达并可以被配体所激活。
Results: the constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45 - 4b基因。
Methods The plasmid transfection mediated by liposomal transfection reagent, HPLC analysis and reverse transcription-PCR analysis were used in this study.
方法采用脂质体介导的质粒转染、流式细胞仪分析、逆转录- PCR及高效液相色谱(HPL C)分析等实验技术方法做有关分析研究。
Methods The plasmid transfection mediated by liposomal transfection reagent, HPLC analysis and reverse transcription-PCR analysis were used in this study.
方法采用脂质体介导的质粒转染、流式细胞仪分析、逆转录- PCR及高效液相色谱(HPL C)分析等实验技术方法做有关分析研究。
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