Now how do you find those colonies on a plate that have the plasmid that you want?
那现在你如何辨别盘子上有我们需要的质粒的菌落呢？
Procyclic trypanosomes were electroporated with linearised plasmid DNA and selected for resistance to phleomycin.
用线性化质粒DNA 电穿孔前环锥虫体，筛选其对磷霉素的抗性。
The recombinant plasmid was transformed into E.
用化学法把重组质粒转化到E 。
Plasmid DNA was extracted by alkaline lysis method.
碱裂解法提取质粒。
Used restrict enzyme and PCR to verify the reconstructed plasmid.
用酶切和PCR的方法对重组质粒进行鉴定。
Objective to construct expression plasmid of human motilin receptor.
目的构建人胃动素受体基因的原核表达载体。
Expression products of recombinant plasmid mainly located in nucleus.
重组质粒表达产物定位于细胞核；
The control group was only given equivalent liposomes except plasmid.
对照组只给予等量的脂质体，不加入质粒。
Objective To construct a bait plasmid in bacterial two-hybrid system.
目的构建大肠杆菌双杂交系统的诱饵重组质粒。
Objective To construct recombinant plasmid for expression of chymopapain in E.
目的构建木瓜凝乳蛋白酶的表达载体，并在大肠杆菌中表达。
Transconjugation test showed that the drug resistant gene is located in plasmid.
接合试验的结果证明耐药基因位于质粒上。
Objective: Study on extraction of plasmid DNA with phenol-chloroform from clone.
目的：对用酚-氯仿直接从克隆中提取质粒dna的方法进行了研究。
Objective To explore a procedure for preparation of plasmid DNA for gene therapy.
目的探索基因治疗用质粒DNA的制备工艺。
The relationship of biomass growth and concentration of plasmid was investigated.
研究了包括菌体生长和质粒含量等的关系。
Study on transfering and diffusing of antibiotic resistant plasmid in Shigella spp.
痢疾杆菌耐药性质粒的转移与扩散的研究。
The plasmid was obtained and identified by alkali splitting and gel electrophoresis.
碱裂解法抽提质粒，琼脂糖电泳分析目的质粒。
One of the ways to introduce genes into a bacterium is to introduce a plasmid into it.
其中一个为细菌加入新基因的方法是引入质粒。
But the different dose of plasmid have the same effect on the survival of a random flap.
不同浓度与剂量的质粒对皮瓣的成活的影响无显著性差异。
Results show that carbon source, nitrogen source affected plasmid DNA yield significantly.
实验结果表明：碳源、氮源对质粒dna产量有明显的影响。
In this technique, scientists use the plasmid from a bacterium that causes tumors on plants.
在应用这种技术时，科学家们使用导致植物肿瘤的细菌中的原生质。
Plasmid technology has also been developed for moving targeted genetic material into plants.
细胞质技术还被应用于把目标基因材料转移到植物的过程中。
The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body.
对含人EC-SOD重组质粒的工程菌进行诱导表达形成包涵体。
Supercoiling is necessary for the expression of recombinant plasmid DNA at transcription level.
超螺旋化对于质粒dna在转录水平上的表达是必需的。
In this review, we summarize the current progresses on plasmid biology in the rare actinomycetes.
本文综述目前稀有放线菌中的质粒生物学的研究进展。
Objective to control the quality of mouse nerve growth factor (NGF) DNA plasmid for gene therapy.
目的对小鼠神经生长因子(NGF)基因治疗型DNA质粒进行质量控制。
Objective To construct a recombinant plasmid for expression of rabies virus glycoprotein in COS-7 cells.
目的构建狂犬病病毒糖蛋白基因的真核细胞表达载体，并在COS - 7细胞中表达。
We established the techniques of screening the plasmid expression library and cloning the immunogen genes.
建立了筛选质粒表达文库和克隆免疫原基因的技术方法。
Plasmid and chromosome extraction experiments indicate that the degradation genes of W1 are on chromosome.
W1菌体质粒和染色体提取实验表明，其降解基因位于染色体上。
Switch on the vacuum to draw the solution through the column (The plasmid DNA binds to the column material).
开启真空装置使液体通过管柱（质体DNA会与管柱物质结合）。
Recobinant plasmid PSP1, microorganisms transformed therewith, and method for producing alkaline protease VAPK.
重组质粒PSP1，转化微生物，产生一种碱性蛋白酶vapk的方法。
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