Objective To prepare polyclonal anti-LPS antibody with cross protection against LPS from different bacteria and to screen mimotopes of LPS from cyclic 7-mer phage peptide library.
目的获得具交叉保护性的抗细菌脂多糖(LPS)多克隆抗体与模拟LPS多表位的噬菌体展示环肽克隆。
Here viruses that infect bacteria, called phage, are engineered such that each virus has on its surface coat a unique random peptide.
在这里,称为噬菌体的使细菌感染的病毒,被设计成这样,即每一种病毒表面都涂了一层独特的随机的肽。
Objective:To obtain the mimotope of avian infectious bronchitis virus by phage display peptide library.
目的:利用噬菌体展示肽库技术筛选鸡传染性支气管炎病毒的模拟抗原表位。
These Abs were used for biopanning of a phage-displayed random15- peptide library.
并以此为筛选配基,对噬菌体表面展示的随机15肽库进行亲和筛选。
Phage-displayed peptide library technology is based on expressing recombinant random peptides on the surface of filamentous phage and selecting function peptides.
噬菌体展示技术是将随机多肽文库表达于丝状噬菌体的表面,筛选功能性多肽的一种生物技术。
Objective: to establish a lung cancer phage-peptide library and to screen for biomarkers for early detection of lung cancer.
目的:构建肺癌噬菌体展示肽库,筛选出肺癌早期检测的分子标志群。
The peptide displayed on positive phage could react specifically with the mouse antiserum against natural JEV Ag .
阳性噬菌体展示肽也能与抗天然JEV抗原的鼠血清产生特异性反应。
Objective to further reconstruct the new phage display vector for more effectively displaying the foreign random peptide library.
目的进一步改造噬菌体展示载体,使之能更有效地展示各种外源随机多肽文库。
Objective To screen the mimic antigen epitopes of Schistosoma japonicum from phage display peptide library.
目的从噬菌体肽库筛选日本血吸虫抗原模拟抗原表位。
METHODS: Using CHO cells which high express human CD59 as target cells, the phage 12 peptide library was screened for 5 rounds by competitive binding test.
方法:以高表达人CD59的中国仓鼠卵巢细胞(CHO)为靶细胞,采用竞争结合试验,对噬菌体随机12肽库进行5轮亲和筛选。
Objective: to select the peptide binding to influenza virus neuraminidase from phage display 12 peptide library.
目的:从噬菌体呈现12肽库中筛选与流感病毒神经氨酸酶特异性结合的肽。
Conclusion This peptide displayed on positive phage may mimic partial antigenicity of JEV E protein.
结论筛选的该噬菌体克隆展示肽可模拟JEVE蛋白的部分抗原性。
Objective To obtain the mimic epitope of specific and sensitive diagnostic antigen in schistosomiasis japonica from phage 12-mer peptide library.
目的从噬菌体十二肽库中筛选出特异、灵敏的日本血吸虫病诊断抗原。
RESULTS Short peptide displayed on phage specifically was bound to 2F2 and the binding was inhibited by natural JEV Ag.
结果筛选到的噬菌体能特异地与2F2结合,并且这种结合可被天然抗原所抑制。
AIM: To select the peptide binding to human transferring receptor (hTfR) from phage display 12 peptides library.
目的:从噬菌体呈现1 2肽库中筛选与人转铁蛋白受体结合的肽。
Here, the basic mechanism and the application of phage display random peptide libraries are briefly introduced.
本文简介此类文库的基本作用原理及应用。
The results also show that phage display peptide library is an effective, simple and efficient method to select specific steroid receptors.
同时,本研究所采用的方法也为使用噬菌体随机肽库进行甾体类激素特异性受体(配体)的筛选提供了可靠的实验依据。
This study utilized purified IBV H52 strain to screen specific virus-binding peptides from Ph. D. -12TM phage display peptide library.
本研究使用纯化的鸡传染性支气管炎病毒(IBV),从12肽噬菌体展示随机肽库中筛选特异性结合多肽。
The mAb epitope was also analyzed using peptide phage display technology.
通过噬菌体肽库技术分析抗体所识别的抗原表位。
Methods: Using the influenza A3 split vaccine as the target molecule, peptides that could bind with target molecule were selected from the phage display 12 peptide library by 3 rounds of biopaning.
方法:以甲三型流感病毒裂解疫苗原液为靶分子,经过3轮生物淘选,从噬菌体随机肽库中筛选与之结合的噬菌体。
Methods: Using the influenza A3 split vaccine as the target molecule, peptides that could bind with target molecule were selected from the phage display 12 peptide library by 3 rounds of biopaning.
方法:以甲三型流感病毒裂解疫苗原液为靶分子,经过3轮生物淘选,从噬菌体随机肽库中筛选与之结合的噬菌体。
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