Objective To construct human phage antibody library and produce human monoclonal antibodies.
目的构建人噬菌体抗体组合文库，筛选人单克隆抗体。
Here a membrane bioreactor system was set up, and was used to produce phage antibody for the first time.
本研究建立了一套膜生物反应器系统，并首次用于噬菌体抗体的生产。
Objective To evaluate the reliability of colony PCR in identifying the recombinant clones selected from phage antibody libraries.
目的评价菌落pcr法鉴定噬菌体抗体库阳性重组率的可靠性。
Conclusion Error-prone PCR is an effective and simple method for affinity maturation of antibodies isolated from a phage antibody library.
结论错配pcr结合噬菌体展示技术是提高单链抗体亲和力的一种有效且简便的手段。
Conclusion:Thiocyanate elution is a powerful and effective method to help obtain the Phabs with high affinity from phage antibody library.
结论：硫氰酸盐洗脱法用于噬菌体抗体的筛选有助于成功获得期望的高亲和力抗体。
A model analytical chip was prepared by coupling 4 phage antibody clones from mouse and 1 clone from human to the surface of carboxyl terminated silicon.
将4个鼠源噬菌体抗体克隆和1个人源噬菌体抗体克隆偶联到羧基终止的硅片表面，制成分析型模型芯片。
Conclusion: Human genetic engineering antibody, which is able to react specifically to tetanus toxin, is obtained through screening phage antibody library.
结论：利用噬菌体抗体库技术筛选可得到与破伤风外毒素特异性结合的人抗破伤风类毒素基因工程抗体。
The phage antibody library technology is a novel method with many potential applications, consisting of antibody library and phage surface display technology.
噬菌体抗体库技术是抗体基因文库技术和噬菌体表面展示技术相结合形成的一项新技术与方法，在生物科学领域极具潜力。
In this study, a new method based on immobilized metal affinity chromatography (IMAC) for screening phage antibody library against antigen in solution was reported.
本研究报道一种基于固定化金属亲和层析(IMAC)的噬菌体抗体库液相筛选方法。
We must prove that there is really brain glioma antibody in the blood of patients, then we can establish the base for constructing humanized phage antibody library.
为了构建人源性抗脑胶质瘤抗体库，我们必须先从患者的血液内检测到抗脑胶质瘤抗体，才能为建立人源性噬菌体抗体库奠定基础。
Conclusion Human anti-keratin scFv is obtained from a large phage antibody library, which will benefit to the application of anti-keratin antibody to clinical research.
结论利用噬菌体抗体库技术获得了人源性抗角蛋白单链抗体，为临床应用研究提供了具有更广阔应用前景的人源小分子抗体。
Antibodies against SARS virus were screened by biopanning with immobilized virus antigen. The binding specificity of phage antibody to SARS virus was detected by ELISA.
以固相化的SARS抗原淘筛抗体库，并用ELISA检测噬菌体抗体结合SARS病毒的特异性。
AIM: To obtain in situ pairing of the variable region genes of the heavy and light chains of vitiligo patients' lymphocytes, then construct and screen a human ScFv phage antibody library.
目的克隆白癜风患者淋巴细胞中原始配对的自身抗体基因，构建噬菌体抗体库并筛选针对黑素细胞膜抗原的特异性抗体。
A large human phage antibody library was subjected for panning with leukocytes from healthy donors and leukemia patients to select for specific antibodies against leukocytic surface proteins.
采用正常人和白血病患者的白细胞对人源噬菌体抗体库进行淘选，以获得对两种细胞表面蛋白特异的抗体。
Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed.
以该融合蛋白为固相抗原，对噬菌体抗体库进行3轮淘筛，并对所获阳性克隆进行抗原结合活性测定和DNA序列测定。
Phage displayed technology has become a powerful tool in preparing high affinity antibodies, and antibody library technology paves a way for obtaining high affinity antibodies.
噬菌体展示技术是一种制备高亲和力抗体的有力工具，抗体库技术则为筛选高亲和力抗体提供了良好的平台。
The construction of phage library of scFV antibody.
人源性天然抗体库的构建。
Conclusion: Recombinant T7 phage vaccine expressing xenogenic VEGF can break immunologic tolerance against self-VEGF and induce the producing of specific anti-VEGF antibody.
结论：异种血管内皮生长因子基因重组T7噬菌体疫苗可打破机体对自身vegf的免疫耐受，诱导产生较高水平的特异性抗vegf抗体。
By using combinatorial library and phage display technologies on a hapten-specific antibody we were able to demonstrate that a peripheral carrier imprint indeed exists.
利用组合库和噬菌体展示技术，我们证明半抗原特异性抗体确实存在有外围载体的某些遗迹。
The phage surface display technology is widely used in disease diagnosis, specific antibody, and protein medicine production.
噬菌体表面展示技术广泛应用于疾病诊断、特异性抗体及蛋白药物生产等。
AIM: To construct phage human anti idiotypic antibody library.
目的：构建噬菌体人源抗独特型抗体库。
Objective To produce monoclonal antibody specific for human brain glioma by the phage display technique.
目的采用噬菌体展示技术制备抗人脑胶质瘤单抗。
Objective To prepare polyclonal anti-LPS antibody with cross protection against LPS from different bacteria and to screen mimotopes of LPS from cyclic 7-mer phage peptide library.
目的获得具交叉保护性的抗细菌脂多糖（LPS）多克隆抗体与模拟LPS多表位的噬菌体展示环肽克隆。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
Conclusion Phage surface display technology is a useful method to produce human monoclonal antibody against CEA.
结论噬菌体抗体库技术是研制人源性单抗的有效途径。
Methods The genes encoding antibody variable regions were cloned by RT PCR from hybridoma cells and expressed on bacterial phage surface.
方法利用RTPCR 方法扩增抗体可变区基因并表达于噬菌体表面。
Methods The genes encoding antibody variable regions were cloned by RT PCR from hybridoma cells and expressed on bacterial phage surface.
方法利用RTPCR 方法扩增抗体可变区基因并表达于噬菌体表面。
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